Localization of cellular retinol-binding protein in several rat tissues.
Localization of cellular retinol-binding protein in several rat tissues.
复制标题
细胞视黄醇结合蛋白在几种大鼠组织中的定位。
DOI：
10.1073/pnas.80.21.6586
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Ong,DE
中科院分区：
文献类型：
--
作者：
Porter,SB;Fraker,LD;Chytil,F;Ong,DE
用过氧化物酶-抗过氧化物酶免疫定位技术研究了细胞视黄醇结合蛋白(CRBP)在大鼠肝脏、回肠和附睾部的分布。用纯化的CRBP制备的抗血清可见肝细胞胞浆染色阳性，而用纯化的CRBP吸收的抗血清则未见胞浆染色。回肠黏膜，一种没有检测到CRBP的组织，没有显示阳性染色。附睾头、尾部未见强阳性染色。在主细胞和基底细胞中有表达，而在管周和间质细胞中没有表达。放射免疫分析表明，附睾头内的CRBP定位于附睾头的起始段和近端，这两个区域与精子成熟所必需的因子的合成和分泌有关。结果表明，CRBP在同一细胞类型中的表达可能不同，也可能在同一组织中不同细胞类型之间的表达不同。
The distribution of cellular retinol-binding protein (CRBP) in rat liver, ileum, and epididymis was examined by the peroxidase-antiperoxidase immunolocalization technique. Positive cytoplasmic staining was seen in the liver when antiserum prepared against purified CRBP was used but not when antiserum absorbed with purified CRBP was used. Ileal mucosa, a tissue that contains no detectable CRBP, showed no positive staining. The epididymis showed strong positive staining in the caput but not in the cauda. Staining was present in principal and basal cells but not in peritubular or interstitial cells. Radioimmunoassay revealed that the CRBP within the caput epididymidis was localized in the initial segment and proximal region, areas known to be involved in the synthesis and secretion of factors necessary for sperm maturation. The results demonstrate that the expression of CRBP may vary within the same cell type, as well as between different cell types within the same tissue.