Molecular Evolution of Viruses of the Family Filoviridae Based on 97 Whole-Genome Sequences
Molecular Evolution of Viruses of the Family Filoviridae Based on 97 Whole-Genome Sequences
复制标题
DOI：
10.1128/jvi.03118-12
复制
发表时间：
2013-03-01
影响因子：
5.4
通讯作者：
Nichol, Stuart T.
中科院分区：
文献类型：
--
作者：
Carroll, Serena A.;Towner, Jonathan S.;Nichol, Stuart T.
埃博拉病毒属和马尔堡病毒属（丝状病毒科）中的病毒与人类和非人类灵长类动物中的出血热大规模暴发有关。第一个记录在案的病例发生在45年前的灵长类动物中，但在蝙蝠种群中检测到的病毒遗传多样性的数量，最近被确定为潜在的水库宿主，表明丝状病毒要古老得多。在这里，对97个全基因组序列（其中55个是新报道的）进行了详细的贝叶斯联合系统发育分析，以全面检查丝状病毒科病毒的分子进化速率并估计共同祖先的日期。属于不同物种的病毒的分子进化速率范围从苏丹埃博拉病毒的0.46 × 10（-4）核苷酸替换/位点/年到莱斯顿埃博拉病毒的8.21 × 10（-4）核苷酸替换/位点/年。最近的共同祖先只能追溯到过去50年内的雷斯顿埃博拉病毒和扎伊尔埃博拉病毒物种，并表明这些物种中的病毒可能经历了最近的遗传瓶颈。马尔堡马尔堡病毒和苏丹埃博拉病毒种内的病毒可以进一步追溯，并分别在大约700年和850年前共享最近的共同祖先。对整个家族的研究表明，丝状病毒科的成员，包括最近描述的Lloviu病毒，在大约10，000年前有一个最近的共同祖先。这些数据对于理解丝状病毒在自然历史背景下的演变是有价值的，因为新的储存宿主被确定，并且进一步确定出现，致病性和对公共卫生的持续威胁的机制。
Viruses in the Ebolavirus and Marburgvirus genera (family Filoviridae) have been associated with large outbreaks of hemorrhagic fever in human and nonhuman primates. The first documented cases occurred in primates over 45 years ago, but the amount of virus genetic diversity detected within bat populations, which have recently been identified as potential reservoir hosts, suggests that the filoviruses are much older. Here, detailed Bayesian coalescent phylogenetic analyses are performed on 97 whole-genome sequences, 55 of which are newly reported, to comprehensively examine molecular evolutionary rates and estimate dates of common ancestry for viruses within the family Filoviridae. Molecular evolutionary rates for viruses belonging to different species range from 0.46 x 10(-4) nucleotide substitutions/site/year for Sudan ebolavirus to 8.21 x 10(-4) nucleotide substitutions/ site/year for Reston ebolavirus. Most recent common ancestry can be traced back only within the last 50 years for Reston ebolavirus and Zaire ebolavirus species and suggests that viruses within these species may have undergone recent genetic bottlenecks. Viruses within Marburg marburgvirus and Sudan ebolavirus species can be traced back further and share most recent common ancestors approximately 700 and 850 years before the present, respectively. Examination of the whole family suggests that members of the Filoviridae, including the recently described Lloviu virus, shared a most recent common ancestor approximately 10,000 years ago. These data will be valuable for understanding the evolution of filoviruses in the context of natural history as new reservoir hosts are identified and, further, for determining mechanisms of emergence, pathogenicity, and the ongoing threat to public health.