Diels‐Alder Photoclick Patterning of Extracellular Matrix for Spatially Controlled Cell Behaviors
Diels‐Alder Photoclick Patterning of Extracellular Matrix for Spatially Controlled Cell Behaviors
复制标题
Diels-Alder 细胞外基质光点击图案化，用于空间控制细胞行为
DOI：
10.1002/adma.202303453
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发表时间：
2023
影响因子：
29.4
通讯作者：
Wilson, Maxwell Z.
中科院分区：
文献类型：
--
作者：
Bailey, Sophia J.;Hopkins, Erik;Baxter, Naomi;Whitehead, Isobel;Read de Alaniz, Javier;Wilson, Maxwell Z.
模拟自然组织空间复杂性的策略可以为探索细胞生物学的基本问题提供细胞支架，并为再生医学提供新材料。在这里，作者展示了一个光导图案化平台，该平台使用天然工程细胞外基质（ECM）蛋白作为底物来编程细胞行为。利用了一种光笼化的二烯，它在365 nm光下释放后经历Diels-Alder - based点击化学。通过与市售的马来酰亚胺二烯亲和试剂结合，常见的ECM蛋白（胶原蛋白、纤维连接蛋白基质、层粘连蛋白）与容易购买的功能性小分子和生长因子形成了模式。最后，重点介绍了利用该平台在哺乳动物细胞中空间控制ERK活性和迁移，展示了通过对天然ECM进行模式化学修饰的可编程细胞行为。
Strategies that mimic the spatial complexity of natural tissues can provide cellular scaffolds to probe fundamental questions in cell biology and offer new materials for regenerative medicine. Here, the authors demonstrate a light‐guided patterning platform that uses natural engineered extracellular matrix (ECM) proteins as a substrate to program cellular behaviors. A photocaged diene which undergoes Diels–Alder‐based click chemistry upon uncaging with 365 nm light is utilized. By interfacing with commercially available maleimide dienophiles, patterning of common ECM proteins (collagen, fibronectin Matrigel, laminin) with readily purchased functional small molecules and growth factors is achieved. Finally, the use of this platform to spatially control ERK activity and migration in mammalian cells is highlighted, demonstrating programmable cell behavior through patterned chemical modification of natural ECM.