Human embryonic stem cells possess immune-privileged properties
Human embryonic stem cells possess immune-privileged properties
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DOI：
10.1634/stemcells.22-4-448
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发表时间：
2004-01-01
期刊：
影响因子：
5.2
通讯作者：
Bhatia, M
中科院分区：
文献类型：
--
作者：
Li, L;Baroja, ML;Bhatia, M
人类胚胎干细胞（hESC）预计将成为细胞疗法的主要来源。克服 hESC 排斥的努力包括核转移和收集代表主要组织相容性复合体 (MHC) 多态性最广泛多样性的 hESC 库。令人惊讶的是，对 hESC 的免疫反应尚未经过实验评估。在这里，将 hESC 注射到具有免疫能力的小鼠体内无法诱导免疫反应。未分化和分化的 hESC 无法刺激同种异体原代人类 T 细胞的增殖，并通过独立于分泌因子的细胞机制抑制第三方同种异体树突状细胞介导的 T 细胞增殖。二次再攻击后，与 hESC 共培养的 T 细胞仍对同种异体刺激剂有反应，但在再次暴露于 hESC 时未能增殖。我们的研究表明，hESC 具有独特的免疫特权特征，并为进一步研究 hESC 移植的免疫反应机制提供了前所未有的机会，从而可以避免免疫介导的排斥反应。
Human embryonic stem cells (hESCs) are envisioned to be a major source for cell-based therapies. Efforts to overcome rejection of hESCs include nuclear transfer and collection of hESC banks representing the broadest diversity of major histocompatability complex (MHC) polymorphorisms. Surprisingly, immune responses to hESCs have yet to be experimentally evaluated. Here, injection of hESCs into immune-competent mice was unable to induce an immune response. Undifferentiated and differentiated hESCs failed to stimulate proliferation of alloreactive primary human T cells and inhibited third-party allogeneic dendritic cell-mediated T-cell proliferation via cellular mechanisms independent of secreted factors. Upon secondary rechallenge, T cells cocultured with hESCs were still responsive to allogeneic stimulators but failed to proliferate upon re-exposure to hESCs. Our study demonstrates that hESCs possess unique immune-privileged characteristics and provides an unprecedented opportunity to further investigate the mechanisms of immune response to transplantation of hESCs that may avoid immune-mediated rejection.