Effect of treatment of latent tuberculosis infection on the T cell response to Mycobacterium tuberculosis antigens
Effect of treatment of latent tuberculosis infection on the T cell response to Mycobacterium tuberculosis antigens
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DOI：
10.1086/499311
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发表时间：
2006-02-01
影响因子：
6.4
通讯作者：
Wilkinson, RJ
中科院分区：
文献类型：
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作者：
Wilkinson, KA;Kon, OM;Wilkinson, RJ
大多数潜伏性结核感染 (LTBI) 病例在感染者一生中不会引起症状。因此，对健康结核分枝杆菌感染者的免疫反应进行纵向分析可能有助于深入了解保护性免疫的基础。在一项纵向研究中，我们记录了治疗对 33 名患有 LTBI 的健康人的 T 细胞对结核分枝杆菌抗原反应的影响。 LTBI 的预防性治疗导致几天内产生干扰素 (IFN) 的 T 细胞数量平均增加 1.8 倍 (P = .006)，随后在治疗期结束时减少 (82 +/- 6 天；P = .004)。在 82 +/- 6 Pp 中，T 细胞对任何抗原的反应没有显着的总体变化。 004名对照组（n=8）的患者选择进行放射学随访。使用活结核分枝杆菌菌株 H37Rv 作为致敏个体的酶联免疫斑点测定中的兴奋剂，我们发现异烟肼（而不是利福平）导致产生 IFN-γ 的细胞数量增加。这些结果表明，细菌细胞壁的完整性对于结核分枝杆菌避免被 T 细胞免疫识别很重要，并有利于 LTBI 的动态模型。
Most cases of latent tuberculosis infection (LTBI) do not cause symptoms during the lifetime of the infected person. Longitudinal analysis of the immune response of healthy Mycobacterium tuberculosis-infected people might, therefore, give insight into the basis of protective immunity. In a longitudinal study, we documented the effect that treatment had on the T cell response to M. tuberculosis antigens in 33 healthy people with LTBI. Preventive treatment of LTBI resulted in a 1.8-fold average increase in the numbers of interferon (IFN)-gamma-producing T cells within days (P = .006), followed by a decrease by the end of the treatment period (82 +/- 6 days;P = .004). There was no significant overall change in the T cell response to any antigen in a 82 +/- 6 Pp. 004 control group (n = 8) of patients who elected radiological follow-up. Using live M. tuberculosis strain H37Rv as a stimulant in an enzyme-linked immunospot assay in sensitized individuals, we showed that isoniazid, but not rifampin, led to an increase in the number of IFN-gamma-producing cells. These results suggest that the integrity of the bacterial cell wall is important for M. tuberculosis in avoiding immune recognition by T cells and favor a dynamic model of LTBI.