Creating Ribo-T: (Design, Build, Test)n
Creating Ribo-T: (Design, Build, Test)n
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DOI：
10.1021/acssynbio.5b00218
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发表时间：
2015-11-01
影响因子：
4.7
通讯作者：
Carlson, Erik D.
中科院分区：
文献类型：
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作者：
Carlson, Erik D.
考虑到我们在生活中复杂的设计场景中理性设计的能力相对较差，工程生物学尤其具有挑战性。因此，多次通过工程“设计、构建、测试”循环积累了系统知识，并有望产生成功的设计。在这里，我将讨论我们最近发表的工作背后的工程过程，该工作创造了一个带有连接亚基的核糖体Ribo-T。
Engineering biology is especially challenging given our relatively poor ability to rationally design within life's complex design landscape. Thus, moving through the engineering "design, build, test" cycle multiple times accumulates system knowledge and hopefully yields a successful design. Here I discuss the engineering process behind our recently published work creating a ribosome with tethered subunits, Ribo-T.