Expression of genes of Rhizoctonia solani and the biocontrol Stachybotrys elegans during mycoparasitism of hyphae and sclerotia
Expression of genes of Rhizoctonia solani and the biocontrol Stachybotrys elegans during mycoparasitism of hyphae and sclerotia
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DOI：
10.3852/10-235
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发表时间：
2011-05-01
期刊：
影响因子：
2.8
通讯作者：
Jabaji, Suha
中科院分区：
文献类型：
--
作者：
Chamoun, Rony;Jabaji, Suha
真菌寄生的知识一直集中在拮抗剂如何影响病原体的机制，代谢产物和基因表达。正如微生物拮抗剂使用不同的机制来控制与宿主的相互作用一样，宿主也有不同的反应来抵消拮抗作用。在这项研究中，差异基因表达的8个mycoparasitism诱导的基因和8个主机响应基因进行了监测，在体内之间的相互作用的mycoparasitiselegans和菌丝和菌核的主机，立枯丝核菌超过5天的相互作用。利用真实的时间反转录聚合酶链反应，比较分析表明，菌丝和菌核结构触发不同的表达模式。这些结果表明，可能涉及多个调节机制。一些属于真菌寄生物和宿主的基因的高表达表明，这些基因分别在真菌寄生过程和宿主防御中发挥重要作用。
Knowledge of mycoparasitism has been focused on how antagonists affect pathogens in relation to mechanisms, metabolites and gene expression. Just as microbial antagonists use a diverse arsenal of mechanisms to dominate interactions with hosts, hosts also have diverse responses to counteract antagonism. In this study differential gene expression of eight mycoparasitism-induced genes and eight host-response genes was monitored during in vivo interactions between the mycoparasite Stachybotrys elegans and hyphae and sclerotia of the host, Rhizoctonia solani over 5 d of interaction. Using real time reverse transcription polymerase chain reaction, comparative analyses demonstrated that hyphal and sclerotial structures triggered different expression patterns. These results indicated that multiple regulatory mechanisms might be involved. The high elevated expression of some genes belonging to the mycoparasite and the host suggest that these genes play an important role during the mycoparasitic process and host defense respectively.