Rapid Identification of Candida Species in Candidemia Directly from Blood Samples Using Imperfect Match Probes
Rapid Identification of Candida Species in Candidemia Directly from Blood Samples Using Imperfect Match Probes
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DOI：
10.1038/s41598-020-62276-5
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发表时间：
2020-04-02
影响因子：
4.6
通讯作者：
Kitajima, Isao
中科院分区：
文献类型：
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作者：
Higashi, Yoshitsugu;Niimi, Hideki;Kitajima, Isao
抗真菌血症与高死亡率相关，初始充分的抗真菌治疗可显著降低粗死亡率。我们在此报告了一种快速的方法，可以确定8个念珠菌属的念珠菌血症使用不完全匹配淬灭探针（IM Q-探针）在三个半小时内的全血样本采集。此外，采用反映来自临床分离株的Tm特征与数据库中登记的Tm特征之间的差异的D值，可以快速鉴定适合于IM Q探针鉴定的样品。我们首先使用从不同患者中收集的34个念珠菌菌落对该方法进行了评估，100%（34/34）的鉴定结果与预先鉴定的念珠菌种相匹配。然后，我们用8份全血样本分别与8种不同的念珠菌进行了盲法试验，所有鉴定结果均与预先鉴定的念珠菌正确匹配。最后，使用16个全血样本收集的念珠菌血症患者，我们比较了IM Q-探针方法与文化/测序方法。在总共16份患者样本中，100%（16/16）与培养和测序结果匹配。IM Q-探针方法不仅有望延长念珠菌血症患者的预期寿命，还有助于抗真菌药物的管理。
Candidemia is associated with a high mortality rate, and initial adequate antifungal therapy results in a significant decrease in the crude mortality. We herein report a rapid method that can identify eight Candida species in candidemia using imperfect match quenching probes (IM Q-probes) within three and a half hours of whole blood sample collection. Furthermore, employing the D value, which reflects the difference between the Tm signature from a clinical isolate and that registered in the database, it is possible to quickly identify samples suitable for IM Q-probe identification. We first evaluated the method using 34 Candida colonies collected from different patients, and 100% (34/34) of the identification results matched the preidentified Candida species. We then performed blind tests using eight whole blood samples artificially mixed with eight different Candida species respectively, and all identification results correctly matched the preidentified Candida species. Finally, using 16 whole blood samples collected from candidemia patients, we compared the IM Q-probe method with the culture/sequencing method. Of a total of 16 patient samples, 100% (16/16) matched the culture and sequencing results. The IM Q-probe method is expected to contribute not only to the life expectancy of candidemia patients but also to antifungal stewardship.