Macrophages largely contribute to heterologous anti‐Propionibacterium acnes antibody‐mediated protection from Actinobacillus pleuropneumoniae infection in mice
Macrophages largely contribute to heterologous anti‐Propionibacterium acnes antibody‐mediated protection from Actinobacillus pleuropneumoniae infection in mice
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DOI：
10.1111/1348-0421.12240
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发表时间：
2015-03
影响因子：
2.6
通讯作者：
Qiu-yue Ma;Changjiang Sun;Feng Yang;Lei Wang;Wanhai Qin;X. Xia;Xin Feng;C. Du;J. Gu;W. Han-W
中科院分区：
文献类型：
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作者：
Qiu-yue Ma;Changjiang Sun;Feng Yang;Lei Wang;Wanhai Qin;X. Xia;Xin Feng;C. Du;J. Gu;W. Han-W
胸膜肺炎放线杆菌是急性和慢性胸膜肺炎的病原体。痤疮丙酸杆菌是一种兼性厌氧革兰氏阳性棒状杆菌。我们以前发现抗P。痤疮抗体可以预防A.小鼠胸膜肺炎感染。为了研究巨噬细胞在这一过程中的作用，亲和纯化的抗P.痤疮IgG和抗A抗体。胸膜肺炎IgG用于调理吞噬测定。此外，用痤疮丙酸杆菌血清被动免疫对A.在巨噬细胞缺失的小鼠中测试了胸膜肺炎。结果发现，抗P。痤疮IgG具有与抗A相似的作用。胸膜肺炎IgG（P > 0.05）显著促进A.巨噬细胞介导的胸膜肺炎（P0.05）。此外，在被动免疫抗P抗体的小鼠中，痤疮血清，巨噬细胞耗竭导致更大的A。胸膜肺炎负荷和肺组织中肺炎的更严重的病理学特征比在巨噬细胞充满的小鼠中发生。得出的结论是，巨噬细胞是必不可少的过程中，抗P。痤疮抗体预防A.小鼠胸膜肺炎感染。
Actinobacillus pleuropneumoniae is the causative agent of acute and chronic pleuropneumonia. Propionibacterium acnes is a facultative anaerobic gram‐positive corynebacterium. We have previously found that anti‐P. acnes antibodies can prevent A. pleuropneumoniae infections in mice. To investigate the role of macrophages in this process, affinity‐purified anti‐P. acnes IgG and anti‐A. pleuropneumoniae IgG were used in opsonophagocytosis assays. Additionally, the efficacy of passive immunization with P. acnes serum against A. pleuropneumoniae was tested in macrophage‐depleted mice. It was found that anti‐P. acnes IgG had an effect similar to that of anti‐A. pleuropneumoniae IgG (P > 0.05), which significantly promotes phagocytosis of A. pleuropneumoniae by macrophages (P 0.05). Furthermore, in mice that had been passively immunized with anti‐P. acnes serum, macrophage depletion resulted in a greater A. pleuropneumoniae burden and more severe pathological features of pneumonia in lung tissues than occurred in macrophage‐replete mice. It was concluded that macrophages are essential for the process by which anti‐P. acnes antibody prevents A. pleuropneumoniae infection in mice.