Glucocorticoid-Induced Changes in the Geometry of Osteoclast Resorption Cavities Affect Trabecular Bone Stiffness
Glucocorticoid-Induced Changes in the Geometry of Osteoclast Resorption Cavities Affect Trabecular Bone Stiffness
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糖皮质激素引起的破骨细胞吸收腔几何形状的变化影响小梁骨硬度
DOI：
10.1007/s00223-012-9674-6
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发表时间：
2012
影响因子：
4.2
通讯作者：
G. H. Lenthe
中科院分区：
文献类型：
--
作者：
J. Vanderoost;K. Søe;Ditte Marie Horslev Merrild;J. Delaissé;G. H. Lenthe
骨折风险可通过在骨病理学中观察到的骨微结构变化而增加，例如糖皮质激素诱导的骨质疏松症。再吸收腔呈现这些微结构方面之一。我们最近发现，糖皮质激素（GC）影响的形状的吸收腔。具体而言，我们发现，在GC破骨细胞（OC）的存在下，骨切片上培养更多的沟槽状腔相比，而在没有GC的圆形腔，而总的侵蚀表面保持不变。在这项研究中，我们假设沟状腔与圆形腔相比对骨强度的影响不同。为了验证这一假设，我们在存在和不存在GC的情况下在骨切片上培养OC并量化其尺寸。这些数据被用来模拟OC吸收腔对骨力学性能的影响，使用经验证的梁-壳有限元模型的骨小梁。我们证明了骨吸收腔几何形状的改变足以影响骨能力。在用GC校正增加的EV/BV后，与对照条件的差异不再显著，表明GC诱导的EV/BV增加（与空腔形状密切相关）高度决定了刚度效应。腰椎是受GC诱导的腔体形状变化影响最大的解剖部位。这些发现可能解释了临床观察结果，即GC治疗期间椎骨骨折的患病率比髋部、前臂和其他非椎骨骨折的患病率增加更多。
Bone fracture risk can increase through bone microstructural changes observed in bone pathologies, such as glucocorticoid-induced osteoporosis. Resorption cavities present one of these microstructural aspects. We recently found that glucocorticoids (GCs) affect the shape of the resorption cavities. Specifically, we found that in the presence of GC osteoclasts (OCs) cultured on bone slices make more trenchlike cavities, compared to rather round cavities in the absence of GCs, while the total eroded surface remained constant. For this study, we hypothesized that trenchlike cavities affect bone strength differently compared to round cavities. To test this hypothesis, we cultured OCs on bone slices in the presence and absence of GC and quantified their dimensions. These data were used to model the effects of OC resorption cavities on bone mechanical properties using a validated beam-shell finite element model of trabecular bone. We demonstrated that a change in the geometry of resorption cavities is sufficient to affect bone competence. After correcting for the increased EV/BV with GCs, the difference to the control condition was no longer significant, indicating that the GC-induced increase in EV/BV, which is closely related to the shape of the cavities, highly determines the stiffness effect. The lumbar spine was the anatomic site most affected by the GC-induced changes on the shape of the cavities. These findings might explain the clinical observation that the prevalence of vertebral fractures during GC treatment increases more than hip, forearm and other nonvertebral fractures.
影响因子：
2.4
作者：
Yeni, YN;Fyhrie, DP
通讯作者：
Fyhrie, DP