Production of Hydrogel Microneedles from Fish Scale Biopolymer
Production of Hydrogel Microneedles from Fish Scale Biopolymer
复制标题
DOI：
10.1007/s10924-019-01426-x
复制
发表时间：
2019-06-01
影响因子：
5.3
通讯作者：
Denloye, Adetokunbo
中科院分区：
文献类型：
--
作者：
Olatunji, Ololade;Denloye, Adetokunbo
这项工作首次提出了一种可扩展的低成本方法，用于使用改进的低温压制方法从鱼鳞衍生的生物聚合物生产水凝胶微针。微针由交联水解胶原蛋白（HC）制成。通过装载葡萄糖酸亚铁来测试微针用于药物装载的适用性。在压缩模式下进行的力分析表明，微针在断裂之前可以承受高达0.136N/微针，这是足以刺穿皮肤的强度。交联的微针在PBS中溶胀至其原始质量的340%，并且在24小时内释放34.5%的负载药物，而非交联的微针在几分钟内溶解。对释放药物的化学测试证实，释放的药物通过与乙酰水杨酸形成有色复合物而保留了其化学结构，并使用紫外光谱法来量化释放的浓度。FTIR分析证实了交联时聚合物的改性结构。所提出的方法允许使用热压法在室温下交联，该热压法可以容易地按比例放大。
This work presents for the first time, a scalable, low cost method for producing hydrogel microneedles from fish scales derived biopolymer using a modified low temperature press method. The microneedles are made from cross-linked hydrolyzed collagen (HC). The suitability of the microneedles for drug loading were tested by loading with Ferrous gluconate. Force analysis carried out in compression mode showed that the microneedles could withstand up to 0.136N per microneedle before fracture which is sufficient strength to pierce the skin. The cross-linked microneedles swelled to 340% of their original mass in PBS and within 24h released 34.5% of the loaded drug, while the non cross-linked microneedles dissolved within minutes. Chemical testing of the released drug confirmed that the drug released retained its chemical structure by forming colored complex with acetylsalicylic acid and UV spectrometry was used to quantify the concentration released. FTIR analysis confirmed the modified structure of the polymer when cross-linked. This proposed method allows cross-linking at room temperature using a thermal press method which can be easily scaled up.