Blue light irradiation-induced oxidative stress in vivo via ROS generation in rat gingival tissue.
Blue light irradiation-induced oxidative stress in vivo via ROS generation in rat gingival tissue.
复制标题
蓝光照射通过大鼠牙龈组织中ROS的产生诱导体内氧化应激。
DOI：
10.1016/j.jphotobiol.2015.07.001
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Yoshino F
中科院分区：
文献类型：
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作者：
Yoshida A;Shiotsu-Ogura Y;Wada-Takahashi S;Takahashi S-S;Toyama T;Yoshino F
据报道，通过对活体的蓝光照射诱导伴随活性氧（ROS）产生的氧化应激。在牙科领域，关于蓝光危害的研究报道有限，主要集中在与牙髓热损伤相关的细胞毒性方面。因此，我们对暴露于蓝光的口腔组织进行了体内研究。通过电子自旋共振光谱法测量了黄素腺嘌呤二核苷酸在蓝光照射下产生的ROS。蓝光照射后，分离Wistar大鼠的腭牙龈。收集的样本进行脂质过氧化和谷胱甘肽的生化分析。蓝光照射产生单线态氧，但N-乙酰基-L-半胱氨酸（NAC）浓度依赖性地显著淬灭单线态氧。蓝光显著加速氧化应激，增加牙龈组织中氧化型谷胱甘肽水平。这些作用也被NAC预给药抑制。结果表明，蓝光照射在牙齿漂白治疗的临床水平可能会增强脂质过氧化反应的诱导氧化应激和消耗大量的细胞内谷胱甘肽。此外，NAC可能是一种有效的补充剂，以保护口腔组织免受蓝光辐射诱导的氧化损伤。
It has been reported that oxidative stress with reactive oxygen species (ROS) generation is induced by blue light irradiation to a living body. Only limited research has been reported in dental field on the dangers of blue light, mostly focusing on cytotoxicity associated with heat injury of dental pulp. We thus performed anin vivostudy on oral tissue exposed to blue light. ROS generated upon blue light irradiation of flavin adenine dinucleotide were measured by electron spin resonance spectroscopy. After blue light irradiation, the palatal gingiva of Wistar rats were isolated. Collected samples were subjected to biochemical analysis of lipid peroxidation and glutathione. Singlet oxygen was generated by blue light irradiation, but was significantly quenched in anN-acetyl-l-cysteine (NAC) concentration-dependent manner. Blue light significantly accelerated oxidative stress and increased the oxidized glutathione levels in gingival tissue. These effects were also inhibited by NAC pre-administration. The results suggest that blue light irradiation at clinical levels of tooth bleaching treatment may enhance lipid peroxidation by the induction of oxidative stress and the consumption of a significant amount of intracellular glutathione. In addition, NAC might be an effective supplement for the protection of oral tissues against blue light irradiation-induced oxidative damage.