Identification of an upstream region that controls the transcription of the human autocrine motility factor receptor.
Identification of an upstream region that controls the transcription of the human autocrine motility factor receptor.
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识别控制人类自分泌运动因子受体转录的上游区域。
DOI：
10.1006/bbrc.1995.2031
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发表时间：
1995
影响因子：
3.1
通讯作者：
Raz,A
中科院分区：
文献类型：
--
作者：
Huang,B;Xie,Y;Raz,A
我们从人胎盘cosmid文库中分离出一个0.7 kb的基因组克隆，其中包含自分泌运动因子受体（hAMFR）编码区5 '末端部分。氯霉素乙酰转移酶（CAT）报告基因检测已确定该区域为hAMFR基因的启动子。通过引物延伸，一个转录起始位点（+1）被定位在ATG起始密码子上游129 bp处。DNA序列分析和CAT分析显示，TATA元件位于-485/-468位置，仅能进行边缘转录（低于总活性的5%）。hAMFR启动子的大部分活性是由重叠于起始位点（+1）的转录启动子（Inr）元件贡献的，该元件独立控制hAMFR基因的转录。
We have isolated from a human placenta cosmid library a 0.7 kb genomic clone that contains the 5′ terminal portion of the autocrine motility factor receptor (hAMFR) coding region. Chloramphenicol Acetyl Transferase (CAT) reporter gene assays have identified this region as the promoter of the hAMFR gene. A single transcription initiation site (+1) has been mapped to 129 bp upstream of the ATG start codon by primer extension. DNA sequence analysis and CAT assay revealed a TATA element at the position -485/-468 which was able to conduct only a marginal transcription (less than 5% of the total activity). The majority of the hAMFR promoter′s activity is contributed by a transcription initiator (Inr) element overlapping the initiation site (+1) which independently controls the transcription of the hAMFR gene.