Photoregulation of Gene Expression with Ligand-Modified Caged siRNAs through Host/Guest Interaction
Photoregulation of Gene Expression with Ligand-Modified Caged siRNAs through Host/Guest Interaction
复制标题
通过宿主/客体相互作用用配体修饰的笼式 siRNA 进行基因表达的光调节
作者：
王强;范新丽;经男男;赵晗;于丽佳;汤新景
小干扰RNA（siRNA）可以通过Argonate 2（Ago 2）诱导的RNA干扰（RNAi）有效沉默靶基因。在空间和时间模式中控制siRNA活性是非常重要的。在不同的掩蔽策略中，光笼可以用于通过具有时空和剂量依赖性分辨率的光照射来调节基因表达。许多不同的笼策略和笼组已被报道用于光激活siRNA基因沉默。在本文中，我们描述了一种新的笼化策略，其通过宿主/客体（包括配体/受体）相互作用增加RISC复合物形成/过程的阻断作用，从而增强笼化siRNA活性的抑制直到光活化。该策略可用作设计笼状siRNA的一般方法，用于外源和内源基因的基因沉默的光调节。
Small interfering RNA (siRNA) can effectively silence target genes through Argonate 2 (Ago2)‐induced RNA interference (RNAi). It is very important to control siRNA activity in both spatial and temporal modes. Among different masking strategies, photocaging can be used to regulate gene expression through light irradiation with spatiotemporal and dose‐dependent resolution. Many different caging strategies and caging groups have been reported for light‐activated siRNA gene silencing. Herein, we describe a novel caging strategy that increases the blocking effect of RISC complex formation/process through host/guest (including ligand/receptor) interactions, thereby enhancing the inhibition of caged siRNA activity until light activation. This strategy can be used as a general approach to design caged siRNAs for the photomodulation of gene silencing of exogenous and endogenous genes.