RELATIONSHIPS BETWEEN NITRATE AND OXYGEN-SUPPLY IN SYMBIOTIC NITROGEN-FIXATION BY WHITE CLOVER
RELATIONSHIPS BETWEEN NITRATE AND OXYGEN-SUPPLY IN SYMBIOTIC NITROGEN-FIXATION BY WHITE CLOVER
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DOI：
10.1093/jxb/37.8.1103
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发表时间：
1986-08-01
影响因子：
6.9
通讯作者：
SKOT, L
中科院分区：
文献类型：
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作者：
MINCHIN, FR;MINGUEZ, MI;SKOT, L
暴露的成熟，白色三叶草（白三叶）品种的成熟，植物。330 mg dm ~（-3）NO_3 ~-N处理8 d，使Blanca单株固氮酶活性下降80%。总的根系呼吸没有显着影响，但其组成部分的分析表明，减少了81%的固氮酶相关的呼吸和340%的增长和维持呼吸增加。固氮酶活性的碳成本（每摩尔C2 H4产生的摩尔CO2呼吸）增加了45%，在曝光期间。根瘤中蔗糖含量下降，但下降的方式与固氮酶活性的变化无关。结核的氧扩散阻力增加了五倍。这种电阻增加的表征表明一个异常的修改的扩散屏障，它的结论是，改变在氧气供应的类菌体参与硝酸盐固氮酶活性的影响。
Exposure of mature, nodulated plants of white clover (Trifolium repens) cv. Blanca to 330 mg dm-3 NO3-N for 8 d caused nitrogenase activity per plant to decrease by 80%. Total nodulated root respiration was not significantly affected but analysis of its components showed an 81% decrease in nitrogenase-linked respiration and a 340% increase in growth and maintenance respiration. Carbon costs of nitrogenase activity (mol CO2 respired per mol C2H4 produced) increased by 45% over the exposure period. Sucrose content of the nodules decreased, but the pattern of decrease did not correlate with that of nitrogenase activity. The oxygen diffusion resistance of the nodules was increased by a factor of five. Characterization of this resistance increase suggests an abnormal modification of the diffusion barrier and it is concluded that alteration in the oxygen supply to the bacteroids is involved in the effect of nitrate on nitrogenase activity.