The hrp gene locus of Pseudomonas solanacearum, which controls the production of a type III secretion system, encodes eight proteins related to components of the bacterial flagellar biogenesis complex
The hrp gene locus of Pseudomonas solanacearum, which controls the production of a type III secretion system, encodes eight proteins related to components of the bacterial flagellar biogenesis complex
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DOI：
10.1111/j.1365-2958.1995.tb02284.x
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发表时间：
1995-03
影响因子：
3.6
通讯作者：
F. Gijsegem;C. Gough;C. Zischek;É. Niqueux;M. Arlat;S. Genin;P. Barberis;Sylvie German;Philippe Castello;C. Boucher
中科院分区：
文献类型：
--
作者：
F. Gijsegem;C. Gough;C. Zischek;É. Niqueux;M. Arlat;S. Genin;P. Barberis;Sylvie German;Philippe Castello;C. Boucher
青枯假单胞菌HRP基因簇的5个转录单位是HR诱导的PopA1蛋白分泌所必需的。其中两个单位1和3的核苷酸序列已被报道。在这里，我们给出了另外三个转录单位，单位2，4和7的核苷酸序列，它们一起被预测为编码15个HRP基因。这使得这5个转录单位编码的HRP蛋白总数达到20个，包括正调控蛋白HrpB和植物相互作用不需要的HPAP。在其他18个蛋白质中，8个属于参与动植物细菌病原体和鞭毛生物发生的III型分泌途径的蛋白质家族，2个与分泌系统相关的蛋白质。在已发现的与青枯病菌HRP蛋白相关的各种蛋白质中，植物病原细菌中的蛋白质包括由HRP基因编码的蛋白质。对于动物病原体的HRP相关蛋白，福氏志贺氏菌和鼠伤寒沙门氏菌的spA和mxi基因以及耶尔森氏菌的ysc基因编码的蛋白参与了III型分泌途径。参与鞭毛发生的蛋白质与青枯病菌的hrp蛋白相关，包括鼠伤寒沙门氏菌、枯草芽孢杆菌和大肠杆菌的fli和fli基因编码的蛋白质，以及胡萝卜欧文氏杆菌的mop基因编码的蛋白质。青枯病菌的HRP蛋白也与费氏根瘤菌参与结瘤专一性的蛋白有关。
Five transcription units of the Pseudomonas solanacearum hrp gene cluster are required for the secretion of the HR‐inducing PopA1 protein. The nucleotide sequences of two of these, units 1 and 3, have been reported. Here, we present the nucleotide sequence of the three other transcription units, units 2, 4 and 7, which are together predicted to code for 15 hrp genes. This brings the total number of Hrp proteins encoded by these five transcription units to 20, including HrpB, the positive regulatory protein, and HpaP, which is apparently not required for plant interactions., Among the 18 other proteins, eight belong to protein families regrouping proteins involved in type III secretion pathways in animal and plant bacterial pathogens and in flagellum biogenesis, while two are related solely to proteins involved in secretion systems. For the various proteins found to be related to P. solanacearum Hrp proteins, those in plant‐pathogenic bacteria include proteins encoded by hrp genes. For Hrp‐related proteins of animal pathogens, those encoded by the spa and mxi genes of Shigella flexneri and of Salmonella typhimurium and by the ysc genes of Yersinia are involved in type III secretion pathways. Proteins involved in flagellum biogenesis, which are related to Hrp proteins of P. solanacearum, include proteins encoded by fli and fli genes of S. typhimurium, Bacillus subtils and Escherichia coli and by mop genes of Erwinia carotovora. P. solanacearum Hrp proteins were also found to be related to proteins of Rhizobium fredii involved in nodulation specificity.