Molecular Basis of Neuroimmune Interaction in an In Vitro Coculture Approach
Molecular Basis of Neuroimmune Interaction in an In Vitro Coculture Approach
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DOI：
10.1038/cmi.2008.31
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发表时间：
2008-08
影响因子：
24.1
通讯作者：
M. Nakanishi;T. Furuno
中科院分区：
文献类型：
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作者：
M. Nakanishi;T. Furuno
几年前，来自名古屋、东京和汉密尔顿的一组研究人员开发了一种独特的技术，利用共焦激光扫描荧光显微镜研究神经免疫相互作用。它依赖于通过使用体外共培养皿创建粘附环境来引导免疫和神经细胞相互作用。通过他们的技术，他们能够研究神经细胞如何与免疫细胞（肥大细胞和 T 淋巴细胞）沟通的机制细节，反之亦然。他们表明，神经肥大细胞通讯可以在没有中间转导细胞的情况下发生，并且通过 NK-1 受体操作的神经肽 P 物质是这种通讯的可溶性因子。此外，最近他们还发现，活化的肥大细胞释放的 ATP 介导神经细胞的活化。此外，利用他们的技术，名古屋的研究小组能够研究神经肥大细胞相互作用的分子机制的细节。 N-钙粘蛋白和 CADM1（细胞粘附分子 1）似乎主要介导附着并促进肥大细胞和神经之间的通讯。它将为基于神经免疫相互作用的疾病（如神经源性炎症、肠道疾病、哮喘和自身免疫性疾病）带来新的治疗方式。
A team of researchers from Nagoya, Tokyo and Hamilton developed a unique technique for studying neuroimmune interaction with confocal laser scanning fluorescence microscopy several years ago. It relies on guiding immune and nerve cell interaction by creating an adhesive environment using an in vitro coculture dish. With their technique, they are able to study details of the mechanism of how nerve cells communicate with immune cells (mast cells and T lymphocytes) and vice versa. They showed that nerve-mast cell communication could occur in the absence of an intermediary transducing cell and that the neuropeptide substance P, operating via NK-1 receptors, was a soluble factor of this communication. In addition, recently, they showed that ATP which was released from activated mast cells mediated the activation of nerve cells. Further, with their technique, Nagoya's group was able to study details of the molecular mechanism of nerve-mast cell interaction. N-cadherin and CADM1 (cell adhesion molecule 1) appeared to mediate attachment and promoted the communication between mast cells and nerves predominantly. It would lead to new therapeutic modalities for diseases based on neuroimmune interaction such as neurogenic inflammation, intestinal bowel diseases, asthma, and autoimmune disorders.