Characterization of Neutralizing Epitopes of Varicella-Zoster Virus Glycoprotein H
Characterization of Neutralizing Epitopes of Varicella-Zoster Virus Glycoprotein H
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DOI：
10.1128/jvi.02097-08
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发表时间：
2008-12
影响因子：
5.4
通讯作者：
Y. Akahori;Kazuhiro Suzuki;T. Daikoku;M. Iwai;Y. Yoshida;Y. Asano;Y. Kurosawa;K. Shiraki
中科院分区：
文献类型：
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作者：
Y. Akahori;Kazuhiro Suzuki;T. Daikoku;M. Iwai;Y. Yoshida;Y. Asano;Y. Kurosawa;K. Shiraki
水痘-带状疱疹病毒（VZV）糖蛋白H（gH）是VZV的主要中和靶蛋白，其中和表位具有构象性。10个中和人单克隆抗体的gH被用来映射表位的免疫组化分析，并被分为7个表位组。两个表位组的组合中和功效不是协同的。每个表位部分或完全抵抗伴刀豆球蛋白A阻断gH的糖基化，并且它们的抗体抑制感染的细胞间传播。中和表位包含gH的至少七个独立的蛋白质部分，其充当抑制细胞间扩散的靶标。
ABSTRACT Varicella-zoster virus (VZV) glycoprotein H (gH) is the major neutralization target of VZV, and its neutralizing epitope is conformational. Ten neutralizing human monoclonal antibodies to gH were used to map the epitopes by immunohistochemical analysis and were categorized into seven epitope groups. The combinational neutralization efficacy of two epitope groups was not synergistic. Each epitope was partially or completely resistant to concanavalin A blocking of the glycomoiety of gH, and their antibodies inhibited the cell-to-cell spread of infection. The neutralization epitope comprised at least seven independent protein portions of gH that served as the target to inhibit cell-to-cell spread.