XBAI AND BLNI GENOMIC CLEAVAGE MAPS OF ESCHERICHIA-COLI K-12 STRAIN MG1655 AND COMPARATIVE-ANALYSIS OF OTHER STRAINS
XBAI AND BLNI GENOMIC CLEAVAGE MAPS OF ESCHERICHIA-COLI K-12 STRAIN MG1655 AND COMPARATIVE-ANALYSIS OF OTHER STRAINS
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DOI：
10.1006/jmbi.1993.1401
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发表时间：
1993-07-20
影响因子：
5.6
通讯作者：
WEINSTOCK, GM
中科院分区：
文献类型：
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作者：
PERKINS, JD;HEATH, JD;WEINSTOCK, GM
展示了大肠杆菌 K-12 菌株 MG1655 的完整 XbaI 和 BlnI 裂解图，以及 MG1655 与其他五种 K-12 菌株的物理图的比较。我们使用类似于之前用于 MG1655 的 NotI 和 SfiI 图谱的方法，绘制了 35 个 XbaI 切割位点，生成 35 个片段，大小范围从 8 kb 到 432 kb。 MG1655 图谱对大肠杆菌其他菌株的适用性。通过比较 EMG2、W1485、W3110、AB1157 和 MC4100 与 MG1655 的 NotI、SfiI 和 XbaI 消化模式来评估 coliK-12。菌株之间的变异性（其中一些是通过许多诱变处理步骤而分离的）可以通过脉冲场凝胶电泳轻松检测到。提出了一个模型，根据简单的插入、删除以及在一种情况下的倒位来解释菌株之间的差异。该模型建议插入和删除的大小范围为 1 kb 到 86 kb。一些较大的特征先前已被表征，一些较小的重排可能可以解释先前报道的这些菌株的遗传特征。这些菌株中定位的各种特征被用来将 17BlnI 片段中的 9 个放置在大肠杆菌上。大肠杆菌物理图。通过类似于NotI、SfiI和XbaI图谱所用的杂交实验放置剩余片段。这样，完整的BlnI图就构建完成了。 XbaI 和 BlnI 的切割位点基于 Ruddet al 开发的 EcoMap6 进行坐标分配。此处提供的 MG1655 的 XbaI 和 BlnI 图谱，与之前发布的 MG1655 的 NotI（22 个位点）和 SfiI（31 个位点）图谱相结合，使 MG1655 中映射的稀有限制性位点总数达到 105 个。 比较分析表明该图谱很容易适用于其他 K-12 菌株。
CompleteXbaI andBlnI cleavage maps ofEscherichia coliK-12 strain MG1655 are presented, along with a comparison of the physical map of MG1655 with that of five other K-12 strains. We have mapped 35XbaI cleavage sites generating 35 fragments ranging in size from 8 kb to 432 kb using methods similar to those used previously for theNotI andSfiI maps of MG1655. The applicability of the MG1655 map to other strains ofE. coliK-12 was assessed by comparing theNotI,SfiI andXbaI digestion patterns of EMG2, W1485, W3110, AB1157 and MC4100 with those of MG1655. The variability between strains, some of which are separated by numerous steps of mutagenic treatment, is readily detectable by pulsed-field gel electrophoresis. A model is presented to account for the differences between the strains on the basis of simple insertions, deletions and, in one case, an inversion. Insertions and deletions ranging in size from 1 kb to 86 kb are suggested by this model. Several of the larger features have previously been characterized and some of the smaller rearrangements can potentially account for previously reported genetic features of these strains. The various features localized in these strains were used to place 9 of the 17BlnI fragments on theE. coliphysical map. The remaining fragments were placed by hybridization experiments similar to those used for theNotI,SfiI andXbaI maps. In this way, the completeBlnI map was constructed. The cleavage sites forXbaI andBlnI were assigned coordinates based on EcoMap6 developed by Ruddet al.TheXbaI andBlnI maps of MG1655 presented here, when combined with theNotI (22 sites) andSfiI (31 sites) maps of MG1655 previously published, bring the total number of mapped rare restriction sites in MG1655 to 105. The strain comparison analysis shows that this map is readily adaptable for use with other K-12 strains.