Micromolding of photocrosslinkable hyaluronic acid for cell encapsulation and entrapment
Micromolding of photocrosslinkable hyaluronic acid for cell encapsulation and entrapment
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DOI：
10.1002/jbm.a.30821
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发表时间：
2006-12-01
影响因子：
4.9
通讯作者：
Burdick, Jason A.
中科院分区：
文献类型：
--
作者：
Khademhosseini, Ali;Eng, George;Burdick, Jason A.
水凝胶的微图案化可用于多种应用，包括组织工程、基础生物学研究、诊断和高通量筛选。虽然已经开发了合成聚合物用于这些应用，但天然聚合物如多糖可能对生物样品和基于细胞的装置具有优势，因为它们是体内微环境的天然组分。在这项研究中，我们合成和使用透明质酸（HA）与光反应甲基丙烯酸酯改性，以制造微结构作为微加工器件的功能组件。为了证明这种方法的普遍性，形成了两种类型的微观结构。在第一种方法中，HA微结构被制造并用作对接模板，以使得随后能够在图案的低剪切应力区域内形成细胞微阵列。这些微孔内的细胞保持活力，可以产生球状体，并且可以使用机械破碎回收。在第二种方法中，将细胞直接包封在HA水凝胶内。活胚胎干（ES）细胞或成纤维细胞阵列被包裹在HA水凝胶中，随后可以使用微结构的酶消化来回收。这些结果表明，它是可能的，将光交联HA，天然的，多功能的，可降解的，和生物相容性的生物聚合物，到微机电系统。(c)2006年，Wiley期刊。
Micropatterning of hydrogels is potentially useful for a variety of applications, including tissue engineering, fundamental biological studies, diagnostics, and high-throughput screening. Although synthetic polymers have been developed for these applications, natural polymers such as polysaccharides may have advantages for biological samples and cell-based devices because they are natural components of the in vivo microenvironment. In this study, we synthesized and used hyaluronic acid (HA) modified with photoreactive methacrylates to fabricate microstructures as functional components of microfabricated devices. To demonstrate the universality of this approach, two types of microstructures were formed. In the first approach, HA microstructures were fabricated and used as docking templates to enable the subsequent formation of cell microarrays within low shear stress regions of the patterns. Cells within these microwells remained viable, could generate spheroids, and could be retrieved using mechanical disruption. In the second approach, cells were encapsulated directly within the HA hydrogels. Arrays of viable embryonic stem (ES) cells or fibroblasts were encapsulated within HA hydrogels and could later be recovered using enzymatic digestion of the microstructures. These results demonstrate that it is possible to incorporate photocrosslinkable HA, a natural, versatile, degradable, and biocompatible biopolymer, into microelectromechanical systems. (c) 2006 Wiley Periodicals.