Granulocyte/macrophage-colony-stimulating factor released by adenovirally transduced CT26 cells leads to the local expression of macrophage inflammatory protein 1α and accumulation of dendritic cells at vaccination sites in vivo
Granulocyte/macrophage-colony-stimulating factor released by adenovirally transduced CT26 cells leads to the local expression of macrophage inflammatory protein 1α and accumulation of dendritic cells at vaccination sites in vivo
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腺病毒转导的 CT26 细胞释放的粒细胞/巨噬细胞集落刺激因子导致巨噬细胞炎症蛋白 1α 的局部表达以及树突状细胞在体内疫苗接种部位的积累
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Tsuneo Suzuki
中科院分区：
文献类型：
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作者：
T. Kielian;E. Nagai;A. Ikubo;C. Rasmussen;Tsuneo Suzuki
抗原呈递细胞（APC）在肿瘤特异性免疫应答的产生中起着至关重要的作用。树突状细胞是APC中最有效的，能够激活抗原特异性CD4+和CD8+ T细胞。以前，我们已经描述了如何用产生粒细胞/巨噬细胞集落刺激因子（GM-CSF）的辐照肿瘤细胞接种小鼠，诱导能够保护小鼠免受后续肿瘤攻击的肿瘤特异性免疫。本研究扩展了这些发现，以检查APC浸润疫苗接种部位的类型和负责其招募的趋化因子。从基因工程肿瘤细胞释放的GM-CSF导致树突状细胞在接种部位及其周围的局部积累。定量显示，与β-半乳糖苷酶产生（对照）疫苗接种位点相比，浸润gm - csf产生的树突状细胞数量显著增加了10倍。逆转录/聚合酶链反应、酶联免疫吸附试验和免疫组织化学分析显示，MIP-1α可能是树突状细胞浸润到gm - csf产生组织的原因。这些发现表明GM-CSF可能通过局部产生MIP-1α间接将树突状细胞募集到疫苗接种部位。
Abstract Antigen presenting cells (APC) play an essential role in the generation of tumor-specific immune responses. Dendritic cells are the most potent of APC, capable of activating both antigen-specific CD4+ and CD8+ T cells. Previously, we have described how vaccination of mice with irradiated tumor cells producing granulocyte/macrophage-colony-stimulating factor (GM-CSF) induces tumor-specific immunity capable of protecting mice from a subsequent tumor challenge. The present study extends these findings to examine the types of APC infiltrating vaccination sites and the chemokines responsible for their recruitment. GM-CSF released from genetically engineered tumor cells led to the local accumulation of dendritic cells in and around the vaccination site. Quantification revealed a significant ten-fold increase in the number of dendritic cells infiltrating GM-CSF-producing as opposed to β-galactosidase-producing (control) vaccination sites. Reverse transcription/polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemical analysis of vaccination sites revealed that MIP-1α may be responsible for dendritic cell infiltration into GM-CSF-producing tissues. These findings suggest that GM-CSF may indirectly recruit dendritic cells into vaccination sites through the local production of MIP-1α.
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DOI：
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发表时间：
1997-03
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
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作者：
D. Gabrilovich;Jadranco Corak;I. Ciernik;D. Kavanaugh;D. Carbone
通讯作者：
D. Gabrilovich;Jadranco Corak;I. Ciernik;D. Kavanaugh;D. Carbone
影响因子：
4.3
作者：
Gabrilovich, DI;Ciernik, IF;Carbone, DP
通讯作者：
Carbone, DP
影响因子：
11.2
作者：
C. Armstrong;R. Botella;T. H. Galloway;Nancy B. Murray;Jill Kramp;I. S. Song;J. Ansel
通讯作者：
C. Armstrong;R. Botella;T. H. Galloway;Nancy B. Murray;Jill Kramp;I. S. Song;J. Ansel
使用转导粒细胞-巨噬细胞集落刺激因子而非 B7-1 转导的淋巴瘤细胞进行免疫可激发独特型特异性 T 细胞并产生有效的全身抗肿瘤免疫。
DOI：
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发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Levitsky,HI;Montgomery,J;Ahmadzadeh,M;Staveley-O'Carroll,K;Guarnieri,F;Longo,DL;Kwak,LW
通讯作者：
Kwak,LW