The complete mitochondrial genome sequence of the network pipefish (Corythoichthys flavofasciatus) and the analyses of phylogenetic relationships within the Syngnathidae species
The complete mitochondrial genome sequence of the network pipefish (Corythoichthys flavofasciatus) and the analyses of phylogenetic relationships within the Syngnathidae species
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DOI：
10.1016/j.margen.2014.11.005
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发表时间：
2015-02-01
期刊：
影响因子：
1.9
通讯作者：
Lin, Qiang
中科院分区：
文献类型：
--
作者：
Zhang, Huixian;Zhang, Yanhong;Lin, Qiang
本研究测定了网海龙（Corythoichthys flavofasciatus）（Gasterosteiformes：Syngnathidae）的线粒体基因组序列。有丝分裂基因组是由16,961个核苷酸组成的环状分子，包括13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和一个控制区。基因组的核苷酸组成偏向A + T含量为59.3%。所有tRNA基因具有典型的三叶草二级结构，除了tRNA（Ser（AGY）），其中二氢尿苷臂缺失。梭1130 bp的flavofasciatus控制区含有几个与其他硬骨鱼共有的特征，包括保守的序列块。我们还进行了比较分析网络海龙的有丝分裂基因组的其他海龙科物种的有丝分裂基因组序列，并构建了海龙科物种的系统发育关系的数据集的基础上，包括所有串联的核苷酸序列，除了第三个密码子的位置。分区贝叶斯推断和最大似然分析表明，所有海马物种均形成海马单系群，具有100%PP和BP，但海龙物种并未形成单系群。Colythoichthysflavofasciatus被放置为姐妹关系的海马分支强大的节点支持值。该基因组的获得将为该物种的分子系统学、地理学和遗传分化研究提供重要的信息。(C)2014爱思唯尔有限公司版权所有。
This study determined the mitochondrial genome sequence of the network pipefish (Corythoichthys flavofasciatus) (Gasterosteiformes: Syngnathidae). The mitogenome was a circular molecule consisting of 16,961 nucleotides, including 13 protein-coding genes, 22 tRNA genes, two rRNA genes and a control region. The nucleotide composition of the genome was biased toward A + T content at 59.3%. All tRNA genes had typical cloverleaf secondary structure except for tRNA(Ser (AGY)), in which the dihydrouridine arm was missing. The C. flavofasciatus control region of 1130 bp contained several features common to other teleost, including conserved sequence blocks. We also performed comparative analysis of the network pipefish mitogenome to the available mitogenome sequences of other Syngnathidae species, and phylogenetic relationship of the Syngnathidae species was constructed based on the data sets including all the concatenated nucleotide sequences of the mitogenomes except the third codon positions. Partitioned Bayesian inference and maximum likelihood analyses showed that all seahorse species formed a monophyletic group of Hippocampus with 100% PPs and BPs, but the pipefish species did not form a monophyletic group. Colythoichthysflavofasciatus was placed as a sister relationship to the Hippocampus clade by strong node-supporting values. The availability of mitogenome of the network pipefish will shed light on the molecular systematics, biogeography and genetic differentiation in this species. (C) 2014 Elsevier B.V. All rights reserved.