The leading role of actinobacteria in aerobic cellulose degradation in Sphagnum peat bogs.
The leading role of actinobacteria in aerobic cellulose degradation in Sphagnum peat bogs.
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DOI：
10.1134/s0012496606050243
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发表时间：
2006-09-01
期刊：
影响因子：
--
通讯作者：
Zavarzin, G A
中科院分区：
文献类型：
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作者：
Pankratov, T A;Dedysh, S N;Zavarzin, G A
N和P(参见图1a和1b中环已酰亚胺的变体)。这种现象是由于原核生物能够固定分子氮，从而使导致泥炭泥炭沼泽中纤维素降解的细菌群落具有更自主的功能。在pH值为中性的富营养化生境中，细菌(系统发育类群Firmicuts)或类细胞吞噬细菌(类杆菌类群)是导致细菌纤维素破坏的主要因素。对泥炭泥沼泽中负责相同过程的细菌进行分子鉴定，得到了完全不同的结果。例如，在原泥炭悬浮液中，细菌种群密度为3.9×108个/毫升(表)。在含有纤维素的泥炭中培养55天后，细菌的总密度增加了一个数量级，达到3.4×109个/ml，无论是天然泥炭悬浮液还是富含N、P的纤维分解群落，都没有发现活性芽孢杆菌或细胞吞噬细胞，它们的种群密度低于方法的检测限(105个/ml)，这些细菌对泥炭泥炭中的纤维素降解没有明显的作用。在……里面
N and P in the medium (see the variants with cycloheximide in Figs. 1a and 1b). This phenomenon is accounted for by the fact that prokaryotes are capable of the fixation of molecular nitrogen and, hence, a more autonomous functioning of the bacterial community responsible for cellulose degradation in Sphagnum peat bogs. Bacilli (the phylogenetic group Firmicutes) or Cytophaga-like bacteria (the Bacteroidetes group) are the main agents responsible for bacterial cellulose destruction in eutrophic habitats with neutral pH. Molecular identification of the bacteria responsible for the same process in the Sphagnum peat bogs yielded fundamentally different results. For example, in the original peat suspension, the population density of bacteria was 3.9× 108 cells/ml (table). After incubation in the presence of cellulose for 55 days, the total population density of bacteria increased by an order of magnitude, namely, to 3.4× 109 cells/ml. Active bacillus or cytophage cells were found in neither native peat suspension nor that containing the cellulolytic community enriched with N and P. Their population density was lower than the detection limit of the method (105 cells/ml), and these bacteria played no significant role in cellulose degradation in Sphagnum peat bogs. In