Formation of Osteochondral Organoids from Murine Induced Pluripotent Stem Cells.
Formation of Osteochondral Organoids from Murine Induced Pluripotent Stem Cells.
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从小鼠诱导多能干细胞形成骨软骨类器官。
DOI：
10.1089/ten.tea.2020.0273
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发表时间：
2021
影响因子：
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通讯作者：
Guilak,Farshid
中科院分区：
文献类型：
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作者：
O'Connor,ShannonK;Katz,DakotaB;Oswald,SaraJ;Groneck,Logan;Guilak,Farshid
Osteoarthritis is a debilitating joint disease that is characterized by pathologic changes in both cartilage and bone, potentially involving cross talk between these tissues that is complicated by extraneous factors that are difficult to studyin vivo. To create a model system of these cartilage–bone interactions, we developed an osteochondral organoid from murine induced pluripotent stem cells (iPSCs). Using this approach, we grew organoids from a single cell type through time-dependent sequential exposure of growth factors, namely transforming growth factor β-3 and bone morphogenic protein 2, to mirror bone development through endochondral ossification. The result is a cartilaginous region and a calcified bony region comprising an organoid with the potential for joint disease drug screening and investigation of genetic risk in a patient or disease-specific manner. Furthermore, we also investigated the possibility of the differentiated cells within the organoid to revert to a pluripotent state. It was found that while the cells themselves maintain the capacity for reinduction of pluripotency, encapsulation in the newly formed 3D matrix prevents this process from occurring, which could have implications for future clinical use of iPSCs.
DOI：
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发表时间：
1984-11
期刊：
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影响因子：
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作者：
A. Campbell
通讯作者：
A. Campbell
唾液酸糖缀合物在哺乳动物胰腺腺泡细胞上的分布。
DOI：
10.1177/30.9.6752268
发表时间：
1982
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
Muresan,V;SarrasJr,MP;Jamieson,JD
通讯作者：
Jamieson,JD
用于表征分泌和细胞表面唾液酸糖缀合物的组织化学方法。
DOI：
10.1177/33.5.3989272
发表时间：
1985
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
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作者：
Schulte,BA;Spicer,SS
通讯作者：
Spicer,SS