Lesions in phycoerythrin chromophore biosynthesis in Fremyelia diplosiphon reveal coordinated light regulation of apoprotein and pigment biosynthetic enzyme gene expression
Lesions in phycoerythrin chromophore biosynthesis in Fremyelia diplosiphon reveal coordinated light regulation of apoprotein and pigment biosynthetic enzyme gene expression
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DOI：
10.1105/tpc.015016
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发表时间：
2003-10-01
期刊：
影响因子：
11.6
通讯作者：
Kehoe, DM
中科院分区：
文献类型：
--
作者：
Alvey, RM;Karty, JA;Kehoe, DM
我们已经表征了pebAB操纵子的表达调控，该操纵子编码丝状蓝藻freyella diplosiphon中合成藻红蛋白所需的酶。pebAB操纵子的表达在互补色适应过程中受到调节，互补色适应系统控制着F. diplosiphon中编码几种光收集蛋白的基因的光响应性。我们对pebA突变体的分析表明，尽管在缺乏藻红蛋白的情况下，藻红蛋白及其相关连接蛋白的水平降低，但pebAB和编码藻红蛋白的基因的表达没有明显的调节。相反，这些基因表达的调控是通过最近发现的激活因子CpeR在RNA积累水平上协调的。
We have characterized the regulation of the expression of the pebAB operon, which encodes the enzymes required for phycoerythrobilin synthesis in the filamentous cyanobacterium Fremyella diplosiphon. The expression of the pebAB operon was found to be regulated during complementary chromatic adaptation, the system that controls the light responsiveness of genes that encode several light-harvesting proteins in F. diplosiphon. Our analyses of pebA mutants demonstrated that although the levels of phycoerythrin and its associated linker proteins decreased in the absence of phycoerythrobilin, there was no significant modulation of the expression of pebAB and the genes that encode phycoerythrin. Instead, regulation of the expression of these genes is coordinated at the level of RNA accumulation by the recently discovered activator CpeR.