Spatio-temporal regulation of gene expression defines subpopulations of epidermal stem cells.
Spatio-temporal regulation of gene expression defines subpopulations of epidermal stem cells.
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DOI：
10.1042/bst20200740
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发表时间：
2020-11
影响因子：
3.9
通讯作者：
Maneesha Aruketty;S. Kurinna
中科院分区：
文献类型：
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作者：
Maneesha Aruketty;S. Kurinna
由于表皮干细胞具有独特的生物学特性和在再生治疗中的潜力，对表皮干细胞的研究已经获得了动力。几种转录因子和miRNA已被鉴定为表皮干细胞标志物。然而，表皮干细胞与其后代的分离仍然具有挑战性。单细胞转录组学的引入指出了嵌入角质形成细胞亚群中的表皮干细胞的高度异质性。伪时间推断、RNA速度和细胞熵进一步增强了我们对干细胞的认识，从而发现了表皮干细胞的可塑性。我们探讨了导致表皮干细胞内可塑性特征发现的主要发现以及细胞可塑性在再生医学中的意义。
The search for epidermal stem cells has gained the momentum as they possess unique biological characteristics and a potential in regeneration therapies. Several transcription factors and miRNAs have been identified as epidermal stem cell markers. However, the separation of epidermal stem cells from their progeny remains challenging. The introduction of single-cell transcriptomics pointed to the high degree of heterogeneity in epidermal stem cells imbedded within subpopulations of keratinocytes. Pseudotime inference, RNA velocity, and cellular entropy further enhanced our knowledge of stem cells, allowing for the discovery of the epidermal stem cell plasticity. We explore the main findings that lead to the discovery of the plastic trait within the epidermal stem cells and the implications of cell plasticity in regenerative medicine.