Salvianolate Protects Hepatocytes from Oxidative Stress by Attenuating Mitochondrial Injury.
Salvianolate Protects Hepatocytes from Oxidative Stress by Attenuating Mitochondrial Injury.
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丹参多酚酸盐通过减轻线粒体损伤来保护肝细胞免受氧化应激
DOI：
10.1155/2016/5408705
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Liu CH
中科院分区：
文献类型：
--
作者：
Zhao Q;Peng Y;Huang K;Lei Y;Liu HL;Tao YY;Liu CH
丹参多酚酸盐在我国临床上广泛用于心血管疾病的治疗，但其在肝脏疾病中的应用尚不清楚。本研究旨在探讨丹参多酚酸盐对大鼠肝细胞线粒体的保护作用。为了评价丹参多酚酸盐对损伤的肝细胞的作用，用过氧化氢（H2 O2）诱导α小鼠肝12（AML-12）细胞并用丹参多酚酸盐处理。检测细胞活力、线粒体MitoTracker绿色染色、5，5 ′，6，6 ′-四氯-1，1 ′，3，3 ′-四乙基苯并咪唑-氰化碘（JC-1）水平和细胞色素C（Cyto-C）表达。为了确定丹参多酚酸盐对线粒体损伤的保护作用，雄性Wistar大鼠注射四氯化碳（CCl 4）并给予丹参多酚酸盐（40 mg·kg−1）治疗。检测血清肝功能、肝细胞过氧化损伤指标、肝细胞线粒体HE染色及透射电镜观察。我们的研究结果表明，丹参多酚酸盐有效地保护肝细胞，增加线粒体的活力，并在体外降低Cyto-C的表达。此外，丹参多酚酸盐在体内还能改善肝功能，减轻脂质过氧化指标，减轻线粒体损伤。结论：丹参多酚酸盐在体内外均能有效保护肝细胞免受损伤，其机制可能与其保护肝细胞线粒体免受氧化应激有关。
Salvianolate is widely used to treat angiocardiopathy in clinic in China, but its application in liver diseases remains unclear. Our study aims to investigate the effect of Salvianolate on rat hepatic injury by protecting hepatocyte mitochondria. To evaluate the effects of Salvianolate on injured hepatocytes, alpha mouse liver 12 (AML-12) cells were induced with hydrogen peroxide (H2O2) and treated with Salvianolate. Cell viability and MitoTracker Green for mitochondria and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazole-carbocyanide iodine (JC-1) levels and cytochrome C (Cyto-C) expressions were detected in vitro. To identify the effect of Salvianolate on protecting against mitochondria injury, male Wistar rats were injected with carbon tetrachloride (CCl4) and treated with Salvianolate (40 mg·kg−1). Serum liver function, parameters for peroxidative damage, hematoxylin and eosin (H&E) staining, and transmission electron microscope (TEM) of hepatocyte mitochondria were assayed. Our results showed that Salvianolate effectively protected hepatocytes, increased mitochondria vitality, and decreased Cyto-C expressions in vitro. Besides, Salvianolate alleviated the liver function, attenuated the indicators of peroxidation, and relieved the mitochondria injury in vivo. In conclusion, Salvianolate is effective in protecting hepatocytes from injury in vitro and in vivo, and the mechanism might be related to its protective effect on hepatocyte mitochondria against oxidative stress.