Pharmacological studies on the hypogastric ganglion of the rat and guinea‐pig
Pharmacological studies on the hypogastric ganglion of the rat and guinea‐pig
复制标题
大鼠和豚鼠腹下神经节的药理研究
DOI：
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发表时间：
1972
影响因子：
7.3
通讯作者：
G. A. Bentley
中科院分区：
文献类型：
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作者：
G. A. Bentley
1通过大鼠或豚鼠的下腹神经节的血管系统用盐水溶液灌注制备。将药物注射到灌注流中，并通过输精管的收缩来指示其作用。可以结扎该器官的基部以防止药物通过血管到达平滑肌。为了进行比较，还对大鼠和豚鼠腹下神经-输精管的完全分离的制备物进行了实验，其中输精管被保持在内浴中，因此添加到外浴中的药物只能作用于神经节。试图证明这些制剂中的非烟碱受体。[2]结果表明，灌流的豚鼠腹下神经节会对几种烟碱类兴奋剂重复反应，尽管最终发生了自身脱敏。完全分离的制剂表现类似，但脱敏更快。相反，大鼠神经节，无论是灌注或完全隔离，是显着不敏感的烟碱类兴奋剂药物。3两种动物对非烟碱类兴奋剂的反应均不佳;豚鼠的动物在约33%的情况下对乙酰甲胆碱产生轻微收缩，但对（4-间氯苯基-氨基甲酰氧基）-2-丁炔基三甲基氯化铵（McN-A-343）无反应。而老鼠的情况正好相反。4在已知可使猫上级颈神经节致敏的某些处理后，豚鼠神经节对非烟碱类兴奋剂并没有变得更敏感。这些措施包括节前破伤风，慢性分散，并删除所有钾。然而，在毒扁豆碱存在下，在对碘化二甲基苯基哌嗪（DMPP）产生快速耐受后，以及当用洗涤红细胞悬液灌注制剂时，确实发生了致敏作用。结论：交感神经节的反应因物种不同而不同，并取决于器官是否被灌注或完全分离。
1 Preparations were developed whereby the hypogastric ganglion of the rat or guinea‐pig was perfused through its vasculature with saline solutions. Drugs were injected into the perfusion stream, and their effects were indicated by contractions of the vas deferens. The base of this organ could be ligated to prevent the drugs reaching the smooth muscle via the blood vessels. For comparison, experiments were also performed on fully isolated preparations of the rat and guinea‐pig hypogastric nerve‐vas deferens, where the vas deferens was held in an inner bath, so that drugs added to the outer bath could act only on the ganglion. Attempts were made to demonstrate non‐nicotinic receptors in these preparations. 2 It was shown that the perfused hypogastric ganglion of the guinea‐pig would respond repeatedly to several nicotinic stimulants, though autodesensitization eventually occurred. The fully isolated preparation behaved similarly, but desensitized much more rapidly. In contrast, the rat ganglion, either perfused or fully isolated, was remarkably insensitive to nicotinic stimulant drugs. 3 Neither species responded well to non‐nicotinic stimulants; that from the guinea‐pig gave small contractions to methacholine in about 33% of cases, but did not respond to (4‐m‐chlorophenyl‐carbamoyloxy)‐2‐butynyl trimethylammonium chloride (McN‐A‐343). With the rat, the situation was opposite. 4 The guinea‐pig ganglion did not become more sensitive to non‐nicotinic stimulants after some treatments known to sensitize the cat superior cervical ganglion. These include preganglionic tetanization, chronic decentralization, and removal of all potassium. Sensitization did occur, however, in the presence of physostigmine, after tachyphylaxis to dimethyl phenyl piperazinium iodide (DMPP) had developed, and when the preparation was perfused with a suspension of washed erythrocytes. 5 It is concluded that the responses of sympathetic ganglia vary from one species to another, and according to whether the organs are perfused or fully isolated.