RNASE-H ACTIVITY OF HIV REVERSE TRANSCRIPTASES IS CONFINED EXCLUSIVELY TO THE DIMERIC FORMS
RNASE-H ACTIVITY OF HIV REVERSE TRANSCRIPTASES IS CONFINED EXCLUSIVELY TO THE DIMERIC FORMS
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DOI：
10.1016/0014-5793(92)80172-d
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发表时间：
1992-03-23
期刊：
影响因子：
3.5
通讯作者：
GOODY, RS
中科院分区：
文献类型：
--
作者：
RESTLE, T;MULLER, B;GOODY, RS
一种快速制备确定的底物来监测RNase H活性的方法已经被开发出来。使用该底物，我们详细研究了不同形式的重组HIV-1和HIV-2逆转录酶（RT）的RNase H活性。正如我们在这里报道的，RNase H活性仅与酶的二聚体形式（p51/p66或p66/p66）相关
A method for the rapid preparation of a defined substrate to monitor RNase H activity has been developed. Using this substrate, we have investigated the RNase H activities of the different forms of recombinant HIV-1 and HIV-2 reverse transcriptase (RT) in detail. As we report here, RNase H activity is associated only with the dimeric forms (p51/p66 or p66/p66) of the enzymes