The yeasts Rho1p and Pkc1p regulate the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane
The yeasts Rho1p and Pkc1p regulate the transport of chitin synthase III (Chs3p) from internal stores to the plasma membrane
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DOI：
10.1073/pnas.1834246100
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发表时间：
2003-09-02
影响因子：
11.1
通讯作者：
Schekman, R
中科院分区：
文献类型：
--
作者：
Valdivia, RH;Schekman, R
在细胞应激期间，酿酒酵母增加几丁质和葡聚糖的合成，以加强和修复细胞壁。在这项研究中，我们表明，在细胞应激的条件下，稳态定位的几丁质合成酶III（Chs 3 p）从内部存储（壳聚糖）转移到质膜（PM）。这种再分配发生迅速，需要细胞壁应激反应信号通路的激活剂，G蛋白Rho 1 p和蛋白激酶Pkc 1 p，但不需要细胞完整性反应丝裂原活化蛋白激酶级联。此外，在没有细胞应激的情况下，Rho 1 p或Pkc 1 p的活化形式的表达足以将Chs 3 p重新分配到PM。在缺乏网格蛋白适配器复合物1和Chs 6p的细胞中，Chs 3 p通过旁路途径转运到PM，细胞壁应激不会引起Chs 3 p的动员，这表明Rho 1 p/Pkc 1 p调节Chs 3 p从trans-Golgi网络中退出。动员细胞内水库的Chs 3 p提供了一个新的机会，调查的遗传基础，受管制的囊泡交通。
During cell stress, Saccharomyces cerevisiae increases the synthesis of chitin and glucans to strengthen and repair the cell wall. In this study, we show that under conditions of cell stress, the steady-state localization of chitin synthase III (Chs3p) shifts from internal stores (chitosomes) to the plasma membrane (PM). This redistribution occurs rapidly and requires the activators of the cell wall stress response signaling pathway, the G protein Rho1p, and the protein kinase Pkc1p, but not the cell integrity response mitogen-activated protein kinase cascade. Furthermore, expression of activated forms of Rho1p or Pkc1p, in the absence of cell stress, is sufficient to redistribute Chs3p to the PM. In cells deficient for both the clathrin adaptor complex 1 and Chs6p, where Chs3p is transported to the PM by an alternative bypass pathway, cell wall stress did not cause mobilization of Chs3p, suggesting that Rho1p/Pkc1p regulate Chs3p exit from the trans-Golgi network. The mobilization of an intracellular reservoir of Chs3p presents a novel opportunity to investigate the genetic basis of regulated vesicular traffic.