The Wnt receptor, Lrp5, is expressed by mouse mammary stem cells and is required to maintain the basal lineage.
The Wnt receptor, Lrp5, is expressed by mouse mammary stem cells and is required to maintain the basal lineage.
复制标题
DOI：
10.1371/journal.pone.0006594
复制
发表时间：
2009-08-12
期刊：
影响因子：
3.7
通讯作者：
Alexander CM
中科院分区：
文献类型：
--
作者：
Badders NM;Goel S;Clark RJ;Klos KS;Kim S;Bafico A;Lindvall C;Williams BO;Alexander CM
Ectopic Wnt signaling induces increased stem/progenitor cell activity in the mouse mammary gland, followed by tumor development. The Wnt signaling receptors, Lrp5/6, are uniquely required for canonical Wnt activity. Previous data has shown that the absence of Lrp5 confers resistance to Wnt1-induced tumor development. Here, we show that all basal mammary cells express Lrp5, and co-express Lrp6 in a similar fashion. Though Wnt dependent transcription of key target genes is relatively unchanged in mammary epithelial cell cultures, the absence of Lrp5 specifically depletes adult regenerative stem cell activity (to less than 1%). Stem cell activity can be enriched by >200 fold (over 80% of activity), based on high Lrp5 expression alone. Though Lrp5 null glands have apparent normal function, the basal lineage is relatively reduced (from 42% basal/total epithelial cells to 22%) and Lrp5−/− mammary epithelial cells show enhanced expression of senescence-associated markers in vitro, as measured by expression of p16Ink4a and TA-p63. This is the first single biomarker that has been demonstrated to be functionally involved in stem cell maintenance. Together, these results demonstrate that Wnt signaling through Lrp5 is an important component of normal mammary stem cell function.
登录
查看更多内容
影响因子：
12.3
作者：
Herschkowitz JI;Simin K;Weigman VJ;Mikaelian I;Usary J;Hu Z;Rasmussen KE;Jones LP;Assefnia S;Chandrasekharan S;Backlund MG;Yin Y;Khramtsov AI;Bastein R;Quackenbush J;Glazer RI;Brown PH;Green JE;Kopelovich L;Furth PA;Palazzo JP;Olopade OI;Bernard PS;Churchill GA;Van Dyke T;Perou CM
通讯作者：
Perou CM
DOI：
10.1023/b:jomg.0000037157.94207.33
发表时间：
2004-04-01
影响因子：
2.5
作者：
Brennan, KR;Brown, AMC
通讯作者：
Brown, AMC
影响因子：
64.8
作者：
Janzen, Viktor;Forkert, Randolf;Scadden, David T.
通讯作者：
Scadden, David T.
DOI：
10.1006/bbrc.1998.9545
发表时间：
1998-10-29
影响因子：
3.1
作者：
Dong, Y;Lathrop, W;Chen, D
通讯作者：
Chen, D
影响因子：
3.3
作者：
Bryja, Vitezslav;Andersson, Emma R.;Arenas, Ernest
通讯作者：
Arenas, Ernest