Fibrinogen Protects Against Barrier Dysfunction Through Maintaining Cell Surface Syndecan-1 In Vitro.
Fibrinogen Protects Against Barrier Dysfunction Through Maintaining Cell Surface Syndecan-1 In Vitro.
复制标题
纤维蛋白原通过在体外维持细胞表面 Syndecan-1 来防止屏障功能障碍。
DOI：
10.1097/shk.0000000000001207
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Kozar,RosemaryA
中科院分区：
文献类型：
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作者：
Wu,Feng;Kozar,RosemaryA
背景：我们已经证明，新鲜冰冻血浆(FFP)对失血性休克后肺内皮细胞屏障完整性的保护作用部分是由于内皮细胞syndecan-1的恢复。在本研究中，我们研究了FFP的主要成分纤维蛋白原作为内皮保护剂的作用，并假设纤维蛋白原稳定细胞表面Syndecan-1以恢复内皮屏障的完整性。方法：将肺内皮细胞孵育在FFP、纤维蛋白原或乳酸林格(LR)中，然后用抗Syndecan-1或纤维蛋白原进行免疫染色，评估屏障的完整性。结果：与LR组相比，FFP和Fg组细胞表面Syndecan-1表达增加，屏障完整性增强。生理浓度为2.5 mg/mL的纤维蛋白原足以增加细胞表面Syndecan-1的表达。共定位和免疫共沉淀实验表明，纤维蛋白原与Syndecan-1结合。纤维蛋白原缺陷血浆不能增强SYNDECAN-1的免疫染色，失去其对内皮屏障完整性的保护作用。结论：这些数据提示，在体外，纤维蛋白原与细胞表面Syndecan-1结合，增强内皮屏障的完整性。
Background:We have shown that fresh frozen plasma's (FFP) protection of pulmonary endothelial barrier integrity following hemorrhagic shock is due in part to restoration of endothelial syndecan-1. In the present study, we investigated the role of fibrinogen, a major component of FFP, as an endothelial protector and hypothesize that fibrinogen stabilizes cell surface syndecan-1 to restore endothelial barrier integrity.Methods:Pulmonary endothelial cells were incubated in FFP, fibrinogen, or lactated Ringers (LR) then immunostained with anti-syndecan-1 or fibrinogen and barrier integrity assessed. In some experiments, cells were exposed to fibrinogen depleted plasma.Results:Cell surface syndecan-1 was increased by FFP and fibrinogen compared with LR-treated cells while barrier integrity was augmented by FFP and fibrinogen compared with LR. The physiological concentration of 2.5 mg/mL fibrinogen was sufficient to increase cell surface syndecan-1. Colocalization and co-immunoprecipitation experiments demonstrated that fibrinogen associates with syndecan-1. Fibrinogen-deficient plasma was unable to augment sydnecan-1 immunostaining and lost its endothelial protective effect on barrier integrity.Conclusion:These data suggest that in vitro, fibrinogen associated with cell surface syndecan-1 and enhanced endothelial barrier integrity.