Cell Population Tracking and Lineage Construction with Spatiotemporal Context
Cell Population Tracking and Lineage Construction with Spatiotemporal Context
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DOI：
10.1007/978-3-540-75759-7_36
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发表时间：
2007-10
期刊：
影响因子：
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通讯作者：
Kang Li;Mei Chen;T. Kanade
中科院分区：
文献类型：
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作者：
Kang Li;Mei Chen;T. Kanade
利用相差延时显微镜在体外对细胞群体进行自动视觉跟踪对于定量、系统和高通量地测量细胞行为至关重要。这些测量包括迁移、有丝分裂、凋亡和细胞谱系的时空定量。本文介绍了一种自动化细胞跟踪系统，可以同时跟踪和分析数千个细胞。该系统通过逐帧跟踪编译和时空跟踪链接的循环来执行跟踪，结合了两种跟踪范例的能力。我们将该系统应用于一系列细胞群，包括成体干细胞。该系统实现了83.8%-92.5%的跟踪精度，比以前的工作高出8%。
Automated visual-tracking of cell populationsin vitrousing phase contrast time-lapse microscopy is vital for quantitative, systematic and high-throughput measurements of cell behaviors. These measurements include the spatiotemporal quantification of migration, mitosis, apoptosis, and cell lineage. This paper presents an automated cell tracking system that can simultaneously track and analyze thousands of cells. The system performs tracking by cycling through frame-by-frame track compilation and spatiotemporal track linking, combining the power of two tracking paradigms. We applied the system to a range of cell populations including adult stem cells. The system achieved tracking accuracies in the range of 83.8%–92.5%, outperforming previous work by up to 8%.