Cadherin-17 is required to maintain pronephric duct integrity during zebrafish development
Cadherin-17 is required to maintain pronephric duct integrity during zebrafish development
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DOI：
10.1016/s0925-4773(02)00094-1
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发表时间：
2002-07-01
影响因子：
2.6
通讯作者：
Crosier, P
中科院分区：
文献类型：
--
作者：
Horsfield, J;Ramachandran, A;Crosier, P
我们已经分离出一种斑马鱼钙粘蛋白，它与人LI-钙粘蛋白（CDH 17）是同源的。斑马鱼cdh 17在胚胎发育期仅在原肾管中表达，在幼体发育和成年期仅在中肾中表达。与哺乳动物同源基因一样，cdh 17也在成年斑马鱼的肝脏和肠道中表达。我们发现cdh 17阳性中胚层细胞对造血系统没有贡献。与Cdh 17的细胞粘附作用一致，使用反义吗啉代寡核苷酸耗竭Cdh 17功能损害了原肾管形成过程中的细胞凝聚力。我们的研究结果表明，Cdh 17是必要的，在斑马鱼胚胎发育过程中维持原肾管的完整性。这一发现与哺乳动物CDH 17的作用形成鲜明对比。其似乎不参与肾发育。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
We have isolated a zebrafish cadherin that is orthologous to human LI-cadherin (CDH17). Zebrafish cdh17 is expressed exclusively in the pronephric ducts during embryogenesis, and in the mesonephros during larval development and adulthood. Like its mammalian ortholog, cdh17 is also expressed in liver and intestine in adult zebrafish. We show that cdh17-positive mesodermal cells do not contribute to the hematopoietic system. Consistent with a cell adhesion role for Cdh17, depletion of Cdh17 function using antisense morpholino oligonucleotides compromised cell cohesion during pronephric duct formation. Our results indicate that Cdh17 is necessary for maintaining the integrity of the pronephric ducts during zebrafish embryogenesis. This finding contrasts with the role of mammalian CDH17. which does not appear to be involved in nephric development. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.