Chronic ethanol consumption inhibits repair of dimethylnitrosamine-induced DNA alkylation.
Chronic ethanol consumption inhibits repair of dimethylnitrosamine-induced DNA alkylation.
复制标题
长期摄入乙醇会抑制二甲基亚硝胺诱导的 DNA 烷基化修复。
DOI：
10.1016/s0006-291x(88)80731-9
复制
发表时间：
1988
影响因子：
3.1
通讯作者：
Garro,AJ
中科院分区：
文献类型：
--
作者：
Mufti,SI;Salvagnini,M;Lieber,CS;Garro,AJ
慢性酒精消耗导致DNA修复缺陷。这一点在Sprague-Dawley大鼠中得到了证实，这些大鼠在成对喂食等热量、乙醇或碳水化合物对照饲料4周后注射了14 C标记的二甲基亚硝胺。在给药亚硝胺后36小时内，从每隔一段时间处死的大鼠中分离肝DNA，并测量烷基化鸟嘌呤衍生物的浓度。虽然N7-甲基鸟嘌呤从两个饮食组的DNA中以相同的速率丢失，但06甲基鸟嘌呤（一种促突变损伤）在酒精喂养的动物的DNA中持续更长时间处于更高水平。
Chronic ethanol consumption causes a DNA repair deficiency. This was demonstrated in Sprague-Dawley rats injected with14C-labeled dimethylnitrosamine after being pair-fed isocaloric, ethanol, or carbohydrate control diets for 4 weeks. Hepatic DNA was isolated from rats killed at intervals over a 36 hour period after administration of the nitrosamine and concentrations of alkylated guanine derivatives were measured. While N7-methylguanine was lost at equivalent rates from the DNA of both diet groups, 06methylguanine, a promutagenic lesion, persisted at higher levels for longer periods of time in the DNA from the alcohol-fed animals.