RAPID TISSUE OXYGEN-TENSION MAPPING USING F-19 INVERSION-RECOVERY ECHO-PLANAR IMAGING OF PERFLUORO-15-CROWN-5-ETHER
RAPID TISSUE OXYGEN-TENSION MAPPING USING F-19 INVERSION-RECOVERY ECHO-PLANAR IMAGING OF PERFLUORO-15-CROWN-5-ETHER
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DOI：
10.1002/mrm.1910320112
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发表时间：
1994-07-01
影响因子：
3.3
通讯作者：
SOTAK, CH
中科院分区：
文献类型：
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作者：
DARDZINSKI, BJ;SOTAK, CH
应用~（19）F反转-恢复回波平面成像（IR-EPI）和新型PFC乳剂全氟-15-冠-5-醚（PFC-C5 O），对小鼠肝、脾和放射诱导纤维肉瘤（RIF-1）肿瘤的氧分压空间分布进行了研究。在成像实验前3至7天，允许腹腔内施用的PFC乳剂在肝脏、脾脏和肿瘤中隔离。采集了7个64 x 64 IR-EPI，反转时间（TI）依次增加。使用非线性最小二乘回归算法逐个像素地拟合七个二维矩阵，以求解隔离PFC的弛豫速率R（1）。根据体外校准曲线，根据测量的R（1）计算氧张力（pO（2））。氧张力地图，然后小鼠肝脏和脾脏产生（在2.5分钟），以证明该技术和组织氧合的变化作为呼吸气体（空气和碳（95%O-2 - 5%CO2））的函数。在不到10分钟的时间内获得了RIF-1肿瘤（n = 5）的组织pO（2）图，并研究了当呼吸气体从空气转换为碳时肿瘤pO（2）的变化。将肿瘤pO（2）图的结果与F-19 MR波谱测量结果进行比较，以检查一致性。直方图分析显示，当动物呼吸空气时，RIF-1肿瘤的平均肝脏和脾脏pO（2）分别为43 μ L和26 μ L。肿瘤氧合作为呼吸气体的函数的统计学显著变化通过pO（2）图（6 +/- 2 μ g，P < 0.05）和F-19 MR波谱（13 +/- 3 μ g，P < 0.01）获得，使用Student配对t检验进行评估。
Fluorine-19 inversion-recovery, echo-planar imaging (IR-EPI) was used in conjunction with a new PFC emulsion, perfluoro-15-crown-5-ether, to map the spatial distribution of oxygen tension in murine liver, spleen and radiation induced fibrosarcoma (RIF-1) tumors. Intravenously administered PFC emulsions were allowed to sequester in the liver, spleen, and tumor 3 to 7 days prior to imaging experiments. Seven, 64 x 64 IR-EPIs were acquired with successively increasing inversion times (TI). A nonlinear least-squares regression algorithm was used to fit the seven two-dimensional matrices, on a pixel-by-pixel basis, to solve for the relaxation rate, R(1), of the sequestered PFC. From in vitro calibration curves, the oxygen tension (pO(2)) was calculated from the measured R(1). Oxygen tension maps were then murine liver and spleen were produced (in 2.5 min) to demonstrate the technique and changes in tissue oxygenation as a function of breathing gas (air and carbogen (95% O-2 - 5% CO2)) are presented. Tissue pO(2) maps from RIF-1 tumors (n = 5) were obtained in less than 10 min and changes in tumor pO(2) were studied when the breathing gas was switched from air to carbogen. The results from tumor pO(2) maps were compared with F-19 MR spectroscopy measurements to check for consistency. Histogram analysis yielded an average liver and spleen pO(2) of 43 torr and 26 torr for RIF-1 tumors when the animals were breathing air. Statistically significant changes in tumor oxygenation as a function of breathing gas were obtained from both pO(2) maps (6 +/- 2 torr, P < 0.05) and F-19 MR spectroscopy (13 +/- 3 torr, P < 0.01) as evaluated using the Student's paired t test.