Potential use of copper surfaces to reduce survival of epidemic meticillin-resistant Staphylococcus aureus in the healthcare environment
Potential use of copper surfaces to reduce survival of epidemic meticillin-resistant Staphylococcus aureus in the healthcare environment
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DOI：
10.1016/j.jhin.2005.12.008
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发表时间：
2006-07-01
影响因子：
6.9
通讯作者：
Keevil, C. W.
中科院分区：
文献类型：
--
作者：
Noyce, J. O.;Michels, H.;Keevil, C. W.
流行性耐甲氧西林金黄色葡萄球菌（EMRSA）于20世纪80年代初出现，EMRSA-15和-16是英国最流行的菌株。MRSA在病人之间的传播主要是通过医护人员的手，可能会造成医院环境的污染。本研究的目的是评估铜和黄铜与不锈钢相比，在降低三种MRSA菌株[MRSA (NCTC 10442), EMRSA-1 （NCTC 11939）和EMRSA-16 (NCTC 13143)]风干沉积物的生存能力方面的有效性。将MRSA和EMRSA[10(7)菌落形成单位（CFU）]接种于铜、黄铜或不锈钢片（1 cm × 1 cm）上，在22℃或4℃下孵育不同时间。通过重悬去除的cfu，并在色氨酸大豆琼脂板上镀上，以及用呼吸指示剂荧光色素5-氰基-2,3-二醇四唑染色来测定其活力。在纯铜表面，107种MRSA、EMRSA-1和EMRSA-16分别在22℃下45分钟、60分钟和90分钟被完全杀死。相比之下，不锈钢（304级）在22℃下72小时后检测到这三种菌株的活菌。在4℃下，铜在6小时内完全杀死了这三种菌株。结果表明，与不锈钢相比，铜对MRSA、EMRSA-1和-16的抑菌效果更好。因此，不建议在医院环境中使用不锈钢作为工作表面和门家具。(c) 2006年，医院感染学会。Elsevier Ltd.出版。版权所有。
Epidemic meticillin-resistant Staphylococcus aureus (EMRSA) emerged in the early 1980s with EMRSA-15 and -16 being the most prevalent strains within the UK. MRSA transmission between patients is largely via the hands of healthcare workers, and contamination of the hospital environment may occur. The objective of this study was to evaluate the effectiveness of copper and brass to reduce the viability of air-dried deposits of three MRSA strains [MRSA (NCTC 10442), EMRSA-1 (NCTC 11939) and EMRSA-16 (NCTC 13143)] compared with stainless steel. MRSA and EMRSA [10(7) colony-forming units (CFU)] were inoculated on to coupons (1 cm x 1 cm) of copper, brass or stainless steel and incubated at either 22 C or 4 C for various time periods. Viability was determined by resuspending removed CFUs and plating out on tryptone soy agar plates in addition to staining with the respiratory indicator fluorochrome 5-cyano-2,3-ditolyl tetrazolium. On pure copper surfaces, 107 MRSA, EMRSA-1 and EMRSA-16 were completely killed after 45, 60 and 90 min, respectively, at 22 degrees C. In contrast, viable organisms for all three strains were detected on stainless steel (grade 304) after 72 h at 22 degrees C. At 4 degrees C, complete kill was achieved on copper for all three strains within 6 h. The results demonstrate an antimicrobial effect of copper on MRSA, EMRSA-1 and -16 in contrast to stainless steel. Consequently, the contemporary application of stainless steel in hospital environments for work surfaces and door furniture is not recommended. (c) 2006 The Hospital Infection Society. Published by Elsevier Ltd. All rights reserved.