Production and activity of matrix metalloproteinase-9 on the ocular surface increase in dysfunctional tear syndrome.
Production and activity of matrix metalloproteinase-9 on the ocular surface increase in dysfunctional tear syndrome.
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DOI：
10.1167/iovs.08-2476
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发表时间：
2009-07
影响因子：
4.4
通讯作者：
Pflugfelder SC
中科院分区：
文献类型：
--
作者：
Chotikavanich S;de Paiva CS;Li de Q;Chen JJ;Bian F;Farley WJ;Pflugfelder SC
目的探讨功能障碍性泪液综合征（dysfunctional tear syndrome，dRS）患者眼表基质金属蛋白酶（metalloproteinase，MMP）-9的产生和活性，并确定MMP-9活性与临床参数之间的相关性。招募了46名新诊断的糖尿病患者和18名对照受试者。进行完整的眼表检查。泪液MMP-9活性用1 μL未刺激泪液中的MMP-9活性测定来评估。使用结膜上皮细胞从19例结膜炎患者和16名对照，MMP-9及其调节细胞因子mRNA转录水平进行了半定量实时PCR评价。四个不同严重程度组的MMP-9平均活性均显著高于对照组，为8.39 ± 4.70 ng/mL。DTS 4组的MMP-9活性最高（381.24 ± 142.83 ng/mL），其平均值显著高于其他三组。此外，结膜炎患者结膜上皮细胞中IL-1β、IL-6、TNF-α和TGF-β1 mRNA转录水平显著高于对照组。泪液MMP-9活性与症状严重程度评分、低对比度视力下降、荧光素泪膜破裂时间、角膜和结膜荧光素染色、地形表面规则性指数（SRI）和共聚焦显微镜检查的异常浅层角膜上皮面积百分比显著相关。泪液MMP-9活性在泪液炎患者中显著升高。这种活性与MMP-9及其调节基因的mRNA表达增加相关，并与临床参数密切相关。MMP-9似乎是一个潜在的有用的生物标志物，用于诊断，分类和监测。
To evaluate production and activity of metalloproteinase (MMP)-9 on the ocular surface of patients with dysfunctional tear syndrome (DTS) and determine any correlation between MMP-9 activity and clinical parameters. Forty-six patients with newly diagnosed DTS and 18 control subjects were recruited. Complete ocular surface examinations were performed. Tear MMP-9 activity was assessed with an MMP-9 activity assay in 1 μL of unstimulated tear fluid. Using conjunctival epithelial cells from 19 patients with DTS and 16 controls, levels of MMP-9 and its regulating cytokine mRNA transcripts were evaluated by semiquantitative real-time PCR. Each of four DTS severity–based groups had significantly higher mean MMP-9 activities than did the control group, which was 8.39 ± 4.70 ng/mL. The DTS4 group had the highest MMP-9 activity (381.24 ± 142.83 ng/mL), for which the mean was significantly higher than that of other DTS groups. In addition, patients with DTS had significantly higher levels of IL-1β, IL-6, TNF-α, and TGF-β1 mRNA transcripts in their conjunctival epithelia than did the control subjects. Tear MMP-9 activities showed significant correlation with symptom severity scores, decreased low-contrast visual acuity, fluorescein tear break-up time, corneal and conjunctival fluorescein staining, topographic surface regularity index (SRI), and percentage area of abnormal superficial corneal epithelia by confocal microscopy. Tear MMP-9 activity was significantly higher in patients with DTS. This activity was associated with increased mRNA expression of MMP-9 and its regulating genes and correlated strongly with clinical parameters. MMP-9 appears to be a potentially useful biomarker for diagnosing, classifying, and monitoring DTS.
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影响因子：
2.8
作者：
Behrens, Ashley;Doyle, John J.;Yiu, Samuel C.
通讯作者：
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影响因子：
5.3
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
4.4
作者：
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通讯作者：
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