Analysis of the human immunodeficiency virus-1 RNA packageome.
Analysis of the human immunodeficiency virus-1 RNA packageome.
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DOI：
10.1261/rna.057299.116
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发表时间：
2016-08
期刊：
影响因子：
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通讯作者：
Wolin SL
中科院分区：
文献类型：
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作者：
Eckwahl MJ;Arnion H;Kharytonchyk S;Zang T;Bieniasz PD;Telesnitsky A;Wolin SL
所有逆转录病毒都将细胞RNA包装成病毒体。对小鼠白血病病毒（MLV）的研究表明，这种简单的逆转录病毒所携带的主要宿主细胞RNA是LTR逆转录转座子和非编码RNA（ncRNA）。几类ncRNA似乎在合成后不久被MLV包装，作为tRNA的前体，小核RNA和小核仁RNA都富集在病毒体中。为了确定人类免疫缺陷病毒（HIV-1）包装相似RNA的程度，我们使用高通量测序来表征CEM-SS T淋巴母细胞中产生的感染性HIV-1病毒粒子内的RNA。我们报告说，最丰富的细胞RNA在HIV-1病毒粒子是7SL RNA和转录本从许多不同的和截短的成员的长散置元件（LINE）和短散置元件（SINE）家族的反转录转座子。我们还检测到前体的几个tRNA和小的核RNA以及来自核糖体DNA（rDNA）基因间间隔的转录本。我们发现，包装前tRNA需要核输出受体Exportin 5，表明HIV-1在细胞质中至少招募了一些新产生的ncRNA。总之，我们的工作确定了由HIV-1包装的RNA集，并揭示了HIV-1组装的早期步骤与宿主细胞ncRNA生物合成途径交叉。
All retroviruses package cellular RNAs into virions. Studies of murine leukemia virus (MLV) revealed that the major host cell RNAs encapsidated by this simple retrovirus were LTR retrotransposons and noncoding RNAs (ncRNAs). Several classes of ncRNAs appeared to be packaged by MLV shortly after synthesis, as precursors to tRNAs, small nuclear RNAs, and small nucleolar RNAs were all enriched in virions. To determine the extent to which the human immunodeficiency virus (HIV-1) packages similar RNAs, we used high-throughput sequencing to characterize the RNAs within infectious HIV-1 virions produced in CEM-SS T lymphoblastoid cells. We report that the most abundant cellular RNAs in HIV-1 virions are 7SL RNA and transcripts from numerous divergent and truncated members of the long interspersed element (LINE) and short interspersed element (SINE) families of retrotransposons. We also detected precursors to several tRNAs and small nuclear RNAs as well as transcripts derived from the ribosomal DNA (rDNA) intergenic spacers. We show that packaging of a pre-tRNA requires the nuclear export receptor Exportin 5, indicating that HIV-1 recruits at least some newly made ncRNAs in the cytoplasm. Together, our work identifies the set of RNAs packaged by HIV-1 and reveals that early steps in HIV-1 assembly intersect with host cell ncRNA biogenesis pathways.