Identification and bioinformatics analysis of differentially expressed milk exosomal microRNAs in milk exosomes of heat-stressed Holstein cows
Identification and bioinformatics analysis of differentially expressed milk exosomal microRNAs in milk exosomes of heat-stressed Holstein cows
复制标题
热应激荷斯坦奶牛乳外泌体中差异表达乳外泌体microRNA的鉴定及生物信息学分析
DOI：
10.1007/s10142-021-00814-8
复制
发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Kunlin Chen
中科院分区：
文献类型：
--
作者：
Yue Wang;Jian Fang;Hanfang Zeng;Jifeng Zhong;Huixia Li;Kunlin Chen
在夏季，热应激是奶牛健康受损和产奶量低的主要原因之一。高温会影响奶牛乳腺的乳汁合成和分泌。作为细胞间通讯的分子，乳源性外泌体携带遗传物质、蛋白质和脂质，在奶牛乳腺组织生长和乳汁合成中起着至关重要的作用。本研究旨在探讨热应激奶牛和正常奶牛乳汁外泌体miRNA的表达谱。我们分离并鉴定了牛奶外泌体，以使用小RNA测序筛选差异表达的miRNA。然后，利用TargetScan和米兰达算法预测差异表达的miRNA的推定靶基因，并对差异表达的miRNA靶基因进行GO和KEGG途径富集分析。我们的研究结果表明，215个miRNAs在热应激乳外泌体中显著差异表达，其中1个上调，214个显著下调。GO和KEGG富集分析表明差异表达的miRNA可能在细胞凋亡、自噬和p38 MAPK通路中起作用。qRT-PCR检测证实miRNAs的表达与测序结果一致，进一步验证了miRNAs的特异性作用靶点。总之，牛奶外泌体的miRNA表达谱的变化表明外泌体miRNA在调节奶牛热应激抗性和细胞凋亡中的作用。我们的研究结果表明，乳源性外泌体miRNAs可以提高乳腺对热应激的抵抗力，从而提高奶牛的牛奶合成。
In summer, heat stress is one of the primary reasons for the compromised health and low milk productivity of dairy cows. Hyperthermia affects milk synthesis and secretion in the mammary glands of dairy cows. As molecules for intercellular communication, milk-derived exosomes carry genetic material, proteins, and lipids, playing a crucial role in mammary tissue growth and milk synthesis in dairy cows. The aim of this study was to explore the milk exosomal miRNA profile of heat-stressed and normal Holstein cows. We isolated and identified milk exosomes to screening for differentially expressed miRNAs using small RNA sequencing. Then, TargetScan and miRanda algorithms were used to predict the putative targets of the differentially expressed miRNAs, whereas GO and KEGG pathway enrichment analyses were performed for the differentially expressed miRNA-target genes. Our results showed that 215 miRNAs were significantly differentially expressed in heat-stressed milk exosomes, of which one was upregulated and 214 were significantly downregulated. GO and KEGG enrichment analyses indicated that differentially expressed miRNAs might play a role in apoptosis, autophagy, and the p38 MAPK pathway. qRT-PCR assay verified that the expression of miRNAs was consistent with the sequencing results, warranting further verification of their specific targets of action. In conclusion, changes in the miRNA expression profile of milk exosomes indicated the role of exosomal miRNAs in regulating heat stress resistance and apoptosis in dairy cows. Our results suggested that milk-derived exosomal miRNAs could increase mammary gland resistance to heat stress, thereby enhancing milk synthesis in dairy cows.
登录
查看更多内容
影响因子：
7
作者：
Zhang S;Chen F;Zhang Y;Lv Y;Heng J;Min T;Li L;Guan W
通讯作者：
Guan W
影响因子：
3.8
作者：
Yueying Zheng;Kunlin Chen;Xiao-min Zheng;Hui-Xia Li;Gen-Lin Wang
通讯作者：
Yueying Zheng;Kunlin Chen;Xiao-min Zheng;Hui-Xia Li;Gen-Lin Wang
影响因子：
5
作者：
Wang, Lanfang;Shi, Zhexi;Li, Ping
通讯作者：
Li, Ping
影响因子：
64.5
作者：
Luga, Valbona;Zhang, Liang;Wrana, Jeffrey L.
通讯作者：
Wrana, Jeffrey L.
影响因子：
3.8
作者：
Cai, Mingcheng;Hu, Yongsong;Lai, Songjia
通讯作者：
Lai, Songjia