Proteomic identification of glyceraldehyde 3-phosphate dehydrogenase as an inhibitory target of hydrogen peroxide in Arabidopsis
Proteomic identification of glyceraldehyde 3-phosphate dehydrogenase as an inhibitory target of hydrogen peroxide in Arabidopsis
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DOI：
10.1016/j.plaphy.2005.07.012
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发表时间：
2005-09-01
影响因子：
6.5
通讯作者：
Neill, SJ
中科院分区：
文献类型：
--
作者：
Hancock, JT;Henson, D;Neill, SJ
过氧化氢（H2O2）现在被认为是真核生物的关键信号分子。在植物中，H2O2参与调节气孔关闭、向地性反应、基因表达和细胞程序性死亡。虽然已知几种激酶，如氧化信号诱导1 （ox1）激酶和丝裂原活化蛋白激酶可被外源H2O2激活，但对直接与H2O2反应的蛋白知之甚少。在这里，我们利用蛋白质组学方法，使用基于碘乙酰胺的荧光标记蛋白结合质谱分析，鉴定了拟南芥细胞质部分中可能是H2O2潜在靶标的几种蛋白，其中最突出的是细胞质中甘油醛3-磷酸脱氢酶（cGAPDH; EC 1.2.1.12）。来自拟南芥的cGAPDH在体外被H2O2灭活，这种抑制可以通过随后添加还原性谷胱甘肽（GSH）等还原剂来逆转。最近有研究表明，拟南芥GAPDH在糖酵解的催化作用之外还有其他作用，而在其他系统中，这包括介导活性氧（ROS）信号传导。在这里，我们认为拟南芥中的cGAPDH也可能在植物中介导ROS信号传导中发挥这样的作用。(c) 2005 Elsevier SAS。版权所有。
Hydrogen peroxide (H2O2) is now recognised as a key signalling molecule in eukaryotes. In plants, H2O2 is involved in regulating stomatal closure, gravitropic responses, gene expression and programmed cell death. Although several kinases, such as oxidative signal-inducible 1 (OXI1) kinase and mitogen-activated protein kinases are known to be activated by exogenous H2O2, little is known about the proteins that directly react with H2O2. Here, we utilised a proteomic approach, using iodoacetamide-based fluorescence tagging of proteins in conjunction with mass spectrometric analysis, to identify several proteins that might be potential targets of H2O2 in the cytosolic fraction of Arabidopsis thaliana, the most prominent of which was cytosolic glyceraldehyde 3-phosphate dehydrogenase (cGAPDH; EC 1.2.1.12). cGAPDH from Arabidopsis is inactivated by H2O2 in vitro, and this inhibition is reversible by the subsequent addition of reductants such as reduced glutathione (GSH). It has been suggested recently that Arabidopsis GAPDH has roles outside of its catalysis as part of glycolysis, while in other systems this includes that of mediating reactive oxygen species (ROS) signalling. Here, we suggest that cGAPDH in Arabidopsis might also have such a role in mediating ROS signalling in plants. (c) 2005 Elsevier SAS. All rights reserved.