Arg-Ile-Tyr (RIY) derived from rapeseed protein decreases food intake and gastric emptying after oral administration in mice
Arg-Ile-Tyr (RIY) derived from rapeseed protein decreases food intake and gastric emptying after oral administration in mice
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DOI：
10.1016/j.peptides.2006.03.019
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发表时间：
2006-09-01
期刊：
影响因子：
3
通讯作者：
Yoshikawa, Masaaki
中科院分区：
文献类型：
--
作者：
Marczak, Ewa D.;Ohinata, Kousaku;Yoshikawa, Masaaki
我们先前报道了一种生物活性三肽Arg-Ile-Tyr（RIY），它已被分离作为从油菜籽蛋白的枯草杆菌蛋白酶消化物中的血管紧张素I转化酶的抑制剂，降低血压。在这项研究中，我们还发现，在禁食的ddY雄性小鼠中口服给药后，150 mg/kg剂量的RIY剂量依赖性地减少摄食量。RIY的促凋亡作用可被胆囊收缩素-1CCK-1受体拮抗剂lorglumide阻断。在150 mg/kg剂量下，RIY还降低胃排空率，并且RIY引起的胃排空延迟被lorglumide阻断。而RIY对CCK 1受体无亲和力。总之，RIY通过刺激CCK释放减少食物摄入和胃排空。(c)2006爱思唯尔公司All rights reserved.
We previously reported that a bioactive tripeptide Arg-Ile-Tyr,(RIY), which has been isolated as an inhibitor for angiotensin I-converting enzyme from the subtilisin digest of rapeseed protein, decreased blood pressure. In this study, we also found that RIY dose-dependently decreased food intake at a dose of 150 mg/kg after oral administration in fasted ddY male mice. The anorexigenic action of RIY was blocked by a cholecystokinin-1 CCK1 receptor antagonist, lorglumide. RIY also decreased the gastric emptying rate at a dose of 150 mg/kg and the RIY-induced delay of gastric emptying was blocked by lorglumide. However, RIY had no affinity for CCK1 receptor. Taken together, RIY decreased food intake and gastric emptying by stimulating CCK release. (c) 2006 Elsevier Inc. All rights reserved.