Evidence for two distinct classes of murine B cell growth factors with activities in different functional assays.
Evidence for two distinct classes of murine B cell growth factors with activities in different functional assays.
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DOI：
10.1084/jem.158.3.822
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发表时间：
1983-09-01
期刊：
影响因子：
--
通讯作者：
Dutton RW
中科院分区：
文献类型：
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作者：
Swain SL;Howard M;Kappler J;Marrack P;Watson J;Booth R;Wetzel GD;Dutton RW
在不同的功能测定中比较了几种先前描述的来自许多小鼠单克隆T细胞来源的B细胞生长因子（BCGF）活性。结果表明，有两个不同的类别的BCGF定义的功能活性和来源。BCGF I的原型是（EL 4）BCGF，在正常脾B细胞的增殖中与抗Ig协同作用，但是当硫酸葡聚糖（DXS）而不是抗Ig用于共刺激相同来源的B细胞时，BCGF I没有活性。BCGF I也不能直接刺激BCL 1肿瘤B细胞。与此相反，BCGF II，其原型是（DL）BCGF，表现出相互的活性模式。BCGF Ⅱ不能协同抗Ig共刺激的正常B细胞产生良好的增殖反应。BCGF II的来源也直接刺激（不添加抗Ig或DXS）携带BCL 1肿瘤的小鼠的B细胞。这些结果表明，两种BCGF可能对两种B细胞亚群具有活性，这两种亚群对两种多克隆B细胞活化剂抗Ig和DXS的诱导反应不同。这些不同的反应模式发生在不同的谱系的B细胞和/或在B细胞在不同的分化状态的可能性进行了讨论。
Several previously described B cell growth factor (BCGF) activities from a number of mouse monoclonal T cell sources were compared in different functional assays. The results indicate that there are two distinct classes of BCGF defined by functional activity and source. BCGF I, whose prototype is (EL4)BCGF, synergized with anti-Ig in the proliferation of normal splenic B cells but had no activity when dextran sulfate (DXS), rather than anti-Ig, was used to costimulate the same source of B cells. BCGF I also failed to directly stimulate BCL1 tumor B cells. In contrast, BCGF II, whose prototype is (DL)BCGF, showed a reciprocal pattern of activity. BCGF II failed to synergize with anti-Ig-costimulated normal B cells to give good proliferative responses. Sources of BCGF II also directly stimulated (no anti-Ig or DXS added) B cells of the BCL1 tumor-carrying mice. These results suggest that the two BCGF may have activity on two subsets of B cells that respond differentially to induction with the two polyclonal B cell activators, anti-Ig and DXS. The possibilities that these different patterns of response occur in separate lineages of B cells and/or in B cells in different states of differentiation is discussed.