Circulating giant macrophages as a potential biomarker of solid tumors
Circulating giant macrophages as a potential biomarker of solid tumors
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DOI：
10.1073/pnas.1320198111
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发表时间：
2014-03-04
影响因子：
11.1
通讯作者：
Cristofanilli, Massimo
中科院分区：
文献类型：
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作者：
Adams, Daniel L.;Martin, Stuart S.;Cristofanilli, Massimo
来自原发性肿瘤的肿瘤相关巨噬细胞（TAM）被认为有助于远端转移的循环肿瘤细胞（CTC）接种，但这些过程的机制知之甚少。尽管许多研究都集中在CTC的迁移上，但对像CTC一样来源于肿瘤部位的TAM的关注较少。我们在低流量条件下使用精密微过滤器，从乳腺癌、胰腺癌或前列腺癌患者的外周血中分离出循环癌症相关巨噬细胞样细胞（CAML）。在健康个体中未发现的CAML被发现表达上皮、单核细胞和内皮蛋白标志物，并且被观察到与循环中的CTC结合。这些数据支持了播散性TAM可用作晚期疾病的生物标志物的假设，并表明它们在肿瘤细胞迁移中具有参与作用。
Tumor-associated macrophages (TAMs) derived from primary tumors are believed to facilitate circulating tumor cell (CTC) seeding of distant metastases, but the mechanisms of these processes are poorly understood. Although many studies have focused on the migration of CTCs, less attention has been given to TAMs that, like CTCs, derive from tumor sites. Using precision microfilters under low-flow conditions, we isolated circulating cancer-associated macrophage-like cells (CAMLs) from the peripheral blood of patients with breast, pancreatic, or prostate cancer. CAMLs, which are not found in healthy individuals, were found to express epithelial, monocytic, and endothelial protein markers and were observed bound to CTCs in circulation. These data support the hypothesis that disseminated TAMs can be used as a biomarker of advanced disease and suggest that they have a participatory role in tumor cell migration.