High Prevalence of CTX-M-15-Producing O25b-ST131 Escherichia coli Clone in Bulgarian Hospitals
High Prevalence of CTX-M-15-Producing O25b-ST131 Escherichia coli Clone in Bulgarian Hospitals
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DOI：
10.1089/mdr.2011.0186
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发表时间：
2012-08-01
影响因子：
2.6
通讯作者：
Mitov, Ivan
中科院分区：
文献类型：
--
作者：
Markovska, Rumyana;Schneider, Ines;Mitov, Ivan
根据欧洲抗菌素耐药性监测系统项目结果，保加利亚已成为超广谱β-内酰胺酶（ESBL）生产者比例急剧增加的欧洲国家之一。这项工作的目的是调查产ESBL大肠埃希菌临床分离株在保加利亚的流行病学，收集从七个临床中心在三个城镇，在两个研究期间：2002-2003年和2006-2009年。对193株产ESBL E.大肠杆菌O25 b-ST 131菌株的随机扩增多态性DNA（RAPD）分析、系统发育分型和筛选。分析药敏结果、ESBL分型及耐药决定因素的可转移性。ESBL分型有TEM-139、SHV-12、CTX-M-3和CTX-M-15 4种，以CTX-M-15为主，占88%。RAPD分型结果显示35个类型，其中A型占优势，占65%的菌株。68%的193株分离株属于O25 b-ST 131克隆，属于系统发育群B2，大多数显示RAPD A型（92%），并在所有参与医院中发现。O25 b-ST 131分离株主要产生CTX-M-15（96%），较少产生SHV-12（n = 3）或TEM-139（n = 2）。总之，本研究首次证实了产高度耐B2 O25 b-ST 131 CTX-M-15大肠杆菌的全国范围传播。coli克隆。
According to the European Antimicrobial Resistance Surveillance System project results, Bulgaria has become one of the European countries with dramatically increasing rates of extended-spectrum beta-lactamase (ESBL) producers. The aim of this work was to investigate the epidemiology of ESBL-producing Escherichia coli clinical isolates in Bulgaria, collected from seven clinical centers in three towns, during two study periods: 2002-2003 and 2006-2009. For 193 ESBL-producing E. coli isolates random amplified polymorphic DNA (RAPD) analyses, phylogenetic typing, and screening for O25b-ST131 isolates were carried out. Antimicrobial susceptibility, ESBL-type and transferability of resistance determinants were analyzed. Four different ESBL-types, namely TEM-139, SHV-12, CTX-M-3, and CTX-M-15 were found. CTX-M-15 dominated, being found in 88% of the isolates. RAPD-typing revealed 35 types, among which type A dominated, comprising 65% of the isolates. Sixty-eight percent of the 193 isolates belonged to the O25b-ST131 clone, to the phylogenetic group B2, mostly showed RAPD-type A (92%) and were found in all participating hospitals. O25b-ST131 isolates predominantly produced CTX-M-15 (96%), and less SHV-12 (n = 3) or TEM-139 (n = 2). In conclusion, this study demonstrated for the first time the country-wide dissemination of a highly resistant B2 O25b-ST131 CTX-M-15 producing E. coli clone in Bulgaria.