B52 promotes alternative splicing of Dscam in Chinese mitten crab, Eriocheir sinensis
B52 promotes alternative splicing of Dscam in Chinese mitten crab, Eriocheir sinensis
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B52 促进中华绒螯蟹 Dscam 的选择性剪接
DOI：
10.1016/j.fsi.2019.01.027
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发表时间：
2019-04-01
影响因子：
4.7
通讯作者：
Wang, Qun
中科院分区：
文献类型：
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作者：
Wan, Zhi-Cheng;Li, Dan;Wang, Qun
B52是经典的富含丝氨酸/精氨酸（SR）的蛋白质，其在遗传上是保守的，并且在mRNA成熟（包括选择性剪接）中起重要作用。对中华绒螯蟹（Eriocheir sinensis）唐氏综合征细胞粘附分子（EsDscam）的对接位点、选择子序列和位点控制区进行了鉴定。Dscam的选择性剪接对于产生不同的异构体是必不可少的。我们还从大肠杆菌中分离并鉴定了B52基因。sinensis（EsB 52）。EsB 52的开放阅读框为876 bp，编码291个氨基酸残基，N端有两个RNA识别基序（RRM），C端有一个富含精氨酸/丝氨酸的结构域。每个RRM包含两个简并短亚基序，RNP-1和RNP 2。组织分布分析表明，EsB 52 mRNA的表达是广泛的，在所有测试的组织，尤其是在脑和血细胞中的高。在血细胞中，EsB 52在病原体相关分子模式和细菌刺激后显著上调。此外，EsB 52 RNAi降低了mRNA中Ig 7的包含物的数量，而不是Ig 2或Ig 3。总之，这些发现表明EsB 52作为EsDscam的选择性剪接激活剂。
B52 is a member of the classical serine/arginine (SR)-rich proteins, which are phylogenetically conserved and play significant roles in mRNA maturation, including alternative splicing. In the present study, the docking site, selector sequences and locus control region of the Chinese mitten crab (Eriocheir sinensis) Down syndrome cell adhesion molecule (EsDscam) were identified. Alternative splicing of Dscam is essential to generate different isoforms. We also isolated and characterised the B52 gene from E. sinensis (EsB52). The 876 bp open reading frame of EsB52 encodes a 291 amino acid residue polypeptide, and EsB52 has two RNA recognition motifs (RRMs) at the N-terminus and an arginine/serine-rich domain at the C-terminus. Each RRM contains two degenerate short submotifs, RNP-1 and RNP2. Analysis of tissue distribution revealed that EsB52 mRNA expression was widespread in all tested tissues, and especially high in brain and hemocytes. In hemocytes, EsB52 was upregulated significantly after stimulation with pathogen-associated molecular patterns and bacteria. Furthermore, EsB52 RNAi decreased the number of Ig7 inclusion in mRNA rather than Ig2 or Ig3. Taken together, these findings suggest that EsB52 acts as an alternative splicing activator of EsDscam.