Prospects for tertiary structure prediction of RNA basea on secondary structure information
Prospects for tertiary structure prediction of RNA basea on secondary structure information
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基于二级结构信息的RNA三级结构预测的展望
DOI：
10.1021/ci2003413
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
K.Fukui
中科院分区：
文献类型：
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作者：
S.Yamasaki;S.Nakamura;K.Fukui
我们开发了一种方法，称为RNA组装使用二级结构信息有效（RASSIE），用于预测RNA的三级结构使用已知的二级结构信息。我们尝试了一种基于片段组装的方法，该方法使用基于二级结构的片段库。对于几个典型的目标结构，如茎环，凸起环，和2路路口，我们的方法提供了许多高质量的候选结构在更少的计算时间比以前提出的方法。通过使用高分辨率的势能函数，我们能够从候选结构中选择良好的预测结构。这种高分辨势的高效构象搜索和详细结构评价方法对于RNA的三级结构预测具有潜在的应用价值。
We developed a method, called RNA Assembler using Secondary Structure Information Effectively (RASSIE), for predicting RNA tertiary structures using known secondary structure information. We attempted a fragment assembly-based method that uses a secondary structure-based fragment library. For several typical target structures such as stem-loops, bulge-loops, and 2-way junctions, our method provided numerous good quality candidate structures in less computational time than previously proposed methods. By using a high-resolution potential energy function, we were able to select good predicted structures from candidate structures. This method of efficient conformational search and detailed structure evaluation using high-resolution potential is potentially useful for the tertiary structure prediction of RNA.