Cyclic mechanical stretch augments hyaluronan production in cultured human uterine cervical fibroblast cells
Cyclic mechanical stretch augments hyaluronan production in cultured human uterine cervical fibroblast cells
复制标题
DOI：
10.1093/molehr/gah229
复制
发表时间：
2005-09-01
影响因子：
4
通讯作者：
Fujii, S
中科院分区：
文献类型：
--
作者：
Takemura, M;Itoh, H;Fujii, S
透明质酸（HA）是一种对水分子具有高亲和力的糖胺聚糖，可刺激局部炎症反应。分娩导致宫颈中HA碎片的量急剧增加，从而有助于宫颈管的快速软化和开放，即宫颈成熟。本研究的目的是调查可能参与的周期性扩张所造成的劳动在增加HA生产在宫颈成熟。免疫组化和/或RT-PCR检测从孕妇获得的人宫颈组织样品中的透明质酸合酶（HAS）1、2和3。24、36和48 h的Labour样周期性机械牵张显著增加了培养的人宫颈成纤维细胞（CxF）分泌HA的能力，分别为128.7、151.4和173.2%，同时HAS 1（36、48 h）、HAS 2（24、36和48 h）和HAS 3（48 h）mRNA表达显著增加。循环机械拉伸12，36和48小时增加从CxF细胞分泌的HA的分子大小。总之，周期性的机械性子宫颈牵拉所造成的胎儿在劳动的一部分，可能有助于增加分泌的HA在宫颈成熟的过程中。
Hyaluronan (HA) a glycosaminoglycan with high affinity for water molecules stimulates local inflammatory reactions. Parturition causes a dramatic increase in the amount of HA fragments in the uterine cervix, thereby contributing to a rapid softening as well as opening of the cervical canal, i.e. cervical ripening. The aim of this study was to investigate the possible involvement of cyclic distension caused by labour in the augmentation of HA production during cervical ripening. Immunohistochemistry and/or RT-PCR detected hyaluronan synthase (HAS)1, 2 and 3 in samples of human cervical tissue obtained from pregnant women. Labour-like cyclic mechanical stretch for 24, 36 and 48 h significantly enhanced the secretion of HA, from cultured human uterine cervical fibroblast (CxF) cells, 128.7, 151.4 and 173.2%, respectively, concomitant with a significant augmentation of HAS1 (36, 48 h), HAS2 (24, 36 and 48 h) and HAS3 (48 h) mRNA expression. Cyclic mechanical stretch for 12, 36 and 48 h increased molecular size of the HA secreted from CxF cells. In conclusion, cyclic mechanical stretch of the uterine cervix caused by the presenting part of the fetus in labour may contribute to the increase in the secretion of HA during the process of cervical ripening.