Dicalcin suppresses in?vitro trophoblast attachment in human cell lines
Dicalcin suppresses in?vitro trophoblast attachment in human cell lines
复制标题
双钙星抑制人细胞系体外滋养层附着
DOI：
10.1016/j.bbrc.2021.07.030
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发表时间：
2021
影响因子：
3.1
通讯作者：
Miwa Naofumi
中科院分区：
文献类型：
--
作者：
Saito Ryohei;Satoh Hiromasa;Aoba Kayo;Hirasawa Hajime;Miwa Naofumi
Implantation is a highly organized process that involves an interaction between a competent blastocyst and a receptive uterus. Despite significant research efforts, the molecular mechanisms governing this complex process remain elusive. Here, we investigated the effect of dicalcin, an S100-like Ca2+-binding protein, on the attachment of choriocarcinoma cells (BeWo cells) onto a monolayer of endometrial carcinoma cells (Ishikawa cells). Extracellularly administered dicalcin bound to both BeWo and Ishikawa cells. Pretreatment of BeWo spheroids with dicalcin reduced the attachment ratio of the spheroids onto the monolayer, whereas that of Ishikawa cells showed no apparent change. We identified the partial amino acid sequence of human dicalcin that exhibited maximum suppression for BeWo spheroid attachment. Transmission electron microscopy analysis revealed that the dicalcin-derived peptide caused a dilation of the intercellular junction between BeWo and ISK cells. Peptide treatment of BeWo spheroids downregulated the expression of integrinαvβ3 in BeWo cells, and induced alterations in their phalloidin-staining pattern, as measured by the length of each F-actin fiber and the thickness of the cortical stress fiber. Thus, dicalcin affects reorganization of the intracellular actin meshwork and subsequently the intensity of attachment, functioning as a novel suppressor of implantation.
