Reproductive effects in male rats exposed to diuron
Reproductive effects in male rats exposed to diuron
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DOI：
10.1016/j.reprotox.2006.09.002
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Kempinas, Wilma G.
中科院分区：
文献类型：
--
作者：
Fernandes, Glaura S. A.;Arena, Arielle C.;Kempinas, Wilma G.
敌草隆是一种脲类除草剂，被认为具有非常低的毒性。本研究评价了敌草隆对成年雄性大鼠生殖毒性的几个方面。将敌草隆稀释在玉米油中，并以0、125或250 mg/kg/天的剂量经口灌胃给予18-20只大鼠，持续30天;对照组仅接受玉米油溶剂。在给药期结束时，将每组约一半的动物分配至两个终末评估线之一：（1）生殖器官、肝脏和肾脏重量;测量肝脏和肾脏中敌草隆浓度;血浆睾酮测定;评价每个睾丸的每日精子产量;附睾中的精子数量和精子通过时间;或（2）与接受性雌性同居期间的性行为评估;自然交配后的生育力和妊娠结局;睾丸、附睾、肾脏和肝脏组织病理学;精子形态学。经口敌草隆处理30天后，体重无处理相关变化，但在所有处理大鼠的肝脏中检测到剂量相关敌草隆残留，两组的绝对和相对肝脏重量均增加。处理组和对照组之间在血浆睾酮浓度、每日精子生成参数、附睾中精子储备、精子形态或男性性行为测定组分方面无统计学显著差异。另一方面，diuron给药大鼠窝中的胎仔数量略少于对照大鼠窝。因此，尽管结果并未表明敌草隆暴露导致大鼠的直接雄性生殖毒性，但它们表明应进行额外的研究，以调查对生育力和生殖能力的可能影响。(c)2006年爱思唯尔公司All rights reserved.
Diuron is a ureic herbicide considered to have very low toxicity. The present study evaluated several aspects of reproductive toxicity of diuron in adult male rats. Diuron was diluted in corn oil and administered by oral gavage to groups of 18-20 rats at doses of 0, 125 or 250 mg/kg per day for 30 days; the control group received only the corn oil vehicle. At the end of the treatment period, approximately half the animals from each group were assigned to one of two terminal assessment lines: (1) reproductive organ, liver and kidney weights; measurement of diuron concentrations in liver and kidney; plasma testosterone determinations; evaluation of daily sperm production per testis; sperm number and sperm transit time in the epididymis; or (2) sexual behavior assessment during cohabitation with a receptive female; fertility and pregnancy outcome after natural mating; testicular, epididymal, kidney and liver histopathology; sperm morphology. After 30 days of oral diuron treatment, there were no treatment-related changes in body weights, but dose-related diuron residues were detected in the liver of all treated rats and absolute and relative liver weights were increased in both groups. There were no statistically significant differences between the treated and control groups obtained in plasma testosterone concentrations, or in parameters of daily sperm production, sperm reserves in the epididymis, sperm morphology or measured components of male sexual behavior. On the other hand, the number of fetuses in the litters from diuron-treated rats was slightly smaller than litters from control rats. Therefore, although the results did not indicate that diuron exposure resulted in direct male reproductive toxicity in the rat, they suggest that additional studies should be undertaken to investigate the possible effects on fertility and reproductive performance. (c) 2006 Elsevier Inc. All rights reserved.